RICTOR Mouse Monoclonal Antibody [Clone ID: 7B3]

CAT#: TA336802

Mouse Monoclonal Rictor Antibody (7B3)

Product Images

Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) TA336802 - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Western Blot: Rictor Antibody (7B3) TA336802 - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.

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  Immunohistochemistry-Paraffin: Rictor Antibody (7B3) TA336802 - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.

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  Flow Cytometry: Rictor Antibody (7B3) TA336802 - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).

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  Flow Cytometry: Rictor Antibody (7B3) TA336802 - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

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  Flow Cytometry: Rictor Antibody (7B3) TA336802 - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) TA336802PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

• Flow Cytometry: Rictor Antibody (7B3) TA336802 - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) TA336802AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Flow Cytometry: Rictor Antibody (7B3) TA336802 - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) TA336802AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

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Specifications

Product Data

• Clone Name: 7B3
• Applications: ELISA, FC, ICC/IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 1 ug per million cells, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000
• Reactivities: Human, Mouse, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human RICTOR expressed in E. coli. [UniProt# Q6R327]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 192 kDa
• Gene Name: RPTOR independent companion of MTOR complex 2
• Database Link:
  NP_001272368
  Entrez Gene 78757 MouseEntrez Gene 253260 Human
  UniProt ID Q6R327
• Synonyms: AVO3; hAVO3; PIA
• Note: This RICTOR (7B3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.Use in Immunoprecipitation reported in scientific literature (PMID: 22952709)

Reference Data

• Protein Pathways: mTOR signaling pathway