Ahr Rabbit Polyclonal Antibody

CAT#: TA301733

Rabbit Polyclonal Antibody against Aryl hydrocarbon Receptor

Product Images

Simple Western: AHR Antibody TA301733 - Image shows a specific band for Aryl hydrocarbon receptor in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: AHR Antibody TA301733 - Aryl hydrocarbon Receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Flow Cytometry: AHR Antibody TA301733 - An intracellular stain was performed on U937 cells with TA301733 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

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  Immunohistochemistry: AHR Antibody TA301733 - Staining of Aryl hydrocarbon Receptor in mouse prostate.

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  Immunocytochemistry/Immunofluorescence: AHR Antibody TA301733 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-AHR at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Flow Cytometry: AHR Antibody TA301733 - An intracellular stain was performed on U-87 cells with AHR Antibody TA301733B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

• Immunocytochemistry/Immunofluorescence: AHR Antibody TA301733 - HepG2 cells were fixed for 10 minutes using 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-AHR at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Western Blot: AHR Antibody TA301733 - Detection of AhR in mouse liver cytosol using NB 100-2289.

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Specifications

Product Data

• Applications: ELISA, FC, ICC/IF, IHC, IP, Simple Western, WB
• Recommended Dilution: Flow Cytometry: 2.5 ug/ml, Western Blot: 1:500-1:2000, ELISA: 1:100 - 1:2000, Immunocytochemistry/ Immunofluorescence: 1:500-1:1000, Immunoprecipitation: 1:10-1:500, Immunohistochemistry: 1:100, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:200, Knockdown Validated
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Bacterially expressed human AhR (C-terminus).
• Formulation: Tris-citrate/phosphate buffer, pH 7-8 and 0.1% sodium azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: aryl-hydrocarbon receptor
• Database Link:
  NP_038492
  Entrez Gene 11622 Mouse
  UniProt ID P30561
• Background: The Ah Receptor belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. All of these proteins share an N-terminal sequence of ~200 amino acids termed the PAS domain. This receptor, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene.
• Synonyms: AH-receptor; bHLHe76