B7-2 (CD86) Mouse Monoclonal Antibody [Clone ID: BU63]

CAT#: SM1167LE

B7-2 (CD86) mouse monoclonal antibody, clone BU63, Low Endotoxin

Specifications

Product Data

• Clone Name: BU63
• Applications: FC, FN, IHC, IP, WB
• Recommended Dilution: Flow cytometry: 5 µg/ml.
  Immunoprecipitation.
  Western blot.
  Immunohistochemistry on frozen sections.
  Functional application: Blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: B-lymphoblastoid cell line ARH 77
• Specificity: This antibody reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
• Formulation: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Low Endotoxin
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD86 molecule
• Database Link:
  Entrez Gene 942 Human
  UniProt ID P42081
• Background: CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
• Synonyms: CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1