p21 (CDKN1A) Mouse Monoclonal Antibody [Clone ID: OTI5G1]

CAT#: CF808128

Carrier-free (BSA/glycerol-free) CDKN1A mouse monoclonal antibody, clone OTI5G1 (formerly 5G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN1A (RC201765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN1A (1:2000). Positive lysates LY409213 (100ug) and LC409213 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-CDKN1A monoclonal antibody (1:200).

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  Western blot analysis of extracts (35ug) from 8 cell lines by using anti-CDKN1A monoclonal antibody (1:250).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA808128) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA808128) (1:150)

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Specifications

Product Data

• Clone Name: OTI5G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-164 of human CDKN1A(NP_510867) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.9 kDa
• Gene Name: cyclin dependent kinase inhibitor 1A
• Database Link:
  NP_510867
  Entrez Gene 1026 Human
  UniProt ID P38936
• Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010]
• Synonyms: CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer