BubR1 (BUB1B) Rabbit Polyclonal Antibody

CAT#: AP55089SU-N

BubR1 (BUB1B) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution:

  ELISA.
  Western Blot: 1/200-1/2000.
  Immunohistochemistry: 1/50-1/500.

• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from N-terminal domain of Human BUB1B
• Specificity: Reacts with Human 115 kDa protein.
  Reacts with cleaved protein and cross reacts with Mouse BUB1 protein.
• Formulation: State: Serum
  State: Lyophilized powder
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store the antibody at -20°C.
  Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: BUB1 mitotic checkpoint serine/threonine kinase B
• Database Link:
  Entrez Gene 701 Human
  UniProt ID O60566
• Background: Metaphase checkpoint controls sense abnormalities in chromosome alignment during mitosis and prevent progression to anaphase until proper alignment has been attained. A number of proteins, including Mitotic arrest deficiency protein 2 (MAD2), Budding uninhibited benzimidazole 1 (BUB1) and Budding uninhibited benzimidazole receptor 1 (BUBR1), have been implicated in the metaphase checkpoint control in mammalian cells. BUB1 and BUBR1 both localize to kinetochores during mitosis, suggesting that they play a role in delaying anaphase until all chromosomes achieve correct, bipolar attachment to the spindle. BUB1 and BUBR1 respond differently to spindle dynamics; they are part of a common complex during mitosis and BUB1 and BUBR1 may integrate different 'spindle assembly signals' into a single signal which can then be interpreted by downstream cell cycle regulators.
• Synonyms: Protein SSK1, BUBR1, MAD3L, BUB1 beta