SP1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP53987PU-N

SP1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in K562 cell line lysates (35ug/lane) using SP1 / TSFP1 antibody. (C-term). This demonstrates the SP1 antibody detected the SP1 protein (arrow).

• 

  Immunohistochemistry analysis in human colon carcinoma (Formalin-fixed, Paraffin-embedded) using SP1 / TSFP1 antibody. (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SP1 / TSFP1 antibody for IHC; Clinical relevance has not been evaluated.

• 

  Confocal immunofluorescent analysis with WiDr cells using SP1 / TSFP1 antibody. (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

• 

  Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using SP1 / TSFP1 antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodie. were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 684-715aa) of human SP1 / TSFP1
• Specificity: This antibody recognizes SP1 / TSFP1 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Sp1 transcription factor
• Database Link:
  Entrez Gene 6667 Human
  UniProt ID P08047
• Background: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
• Synonyms: Transcription factor Sp1
• Note: Molecular Weight: 80693 Da

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Huntington's disease, TGF-beta signaling pathway