MSK1 (RPS6KA5) Rabbit Polyclonal Antibody

CAT#: AP06539PU-N

MSK1 (RPS6KA5) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MSK1 antibody at 1/500 dilution Lane 1:Jurkat whole cell lysate Lane 2:Mouse liver tissue lysate Lane 3:Rat liver tissue lysate

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  Immunohistochemistry (IHC) analysis of MSK1 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 331-380 of Human MSK1.
• Specificity: This antibody detects endogenous levels of MSK1 protein.
  (region surrounding Met355)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% (by SDS-PAGE).
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 90 kDa
• Gene Name: ribosomal protein S6 kinase A5
• Database Link:
  Entrez Gene 9252 Human
  UniProt ID O75582
• Background: MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.
• Synonyms: Ribosomal protein S6 kinase alpha-5, RSKL