E2F1 Rabbit Polyclonal Antibody

CAT#: AP06386PU-N

E2F1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of E2F1 Antibody in extracts from HeLa cells treated with Etoposide 25uM 24h.

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  Immunohistochemistry analyzes of E2F1 Antibody in paraffin-embedded Human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human E2F-1.
• Specificity: This antibody detects endogenous levels of E2F1 protein.
  (region surrounding Asp426)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~47 kDa
• Gene Name: E2F transcription factor 1
• Database Link:
  Entrez Gene 13555 MouseEntrez Gene 1869 Human
  UniProt ID Q01094
• Background: E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.
• Synonyms: E2F-1, RBBP3, PBR3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer