APC Rabbit Polyclonal Antibody

CAT#: AP06015PU-N

APC rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of APC antibody at 1/500 dilution Lane 1:Hela cell lysate Lane 2:HEK293T cell lysate Lane 3:Rat testis tissue lysate

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  Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using APC / DP2.5 antibody.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunhistochemistry on Paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the C-terminal of Human APC.
• Specificity: This antibody detects endogenous levels of APC / DP2.5 protein (region surrounding Gln2829).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~310 kDa
• Gene Name: adenomatous polyposis coli
• Database Link:
  Entrez Gene 324 Human
  UniProt ID P25054
• Background: Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
• Synonyms: Adenomatous polyposis coli protein