Caspase 5 (CASP5) Rabbit Polyclonal Antibody

CAT#: AP05927PU-N

Caspase 5 (CASP5) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of whole cell lysate from Ramos, Burkitt's lymphoma deribed B-lymphoblastoid cells probed with Rabbit anti Human caspase-5 at 0.5 (A), 1 (B) and 2 (C) ug/ml

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  Immunocytochemical staining of P815 mouse mastocytoma cells with Rabbit anti Human caspase-5

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1.0 µg/ml - 2.0 µg/ml; detects a band of approximately 48 kDa in Ramos cell lysates. (Predicted precursor MWT 47.8kDa).
  Immuncytochemistry.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A 14 amino acid peptide from the centre of human Caspase-5.
• Specificity: This antibody recognises an epitope located within the internal region of human Caspase-5, a 47kDa cysteine protease, otherwise known as ICH-3.
• Formulation: Phosphate buffered saline containing 0.02% Sodium Azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: caspase 5
• Database Link:
  Entrez Gene 838 Human
  UniProt ID P51878
• Background: Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.
  Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.
  The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (1).
• Synonyms: CASP-5, CASP5, ICH3, ICH-3 protease, TY protease, ICE(rel)-III