VAV2 (SHD Region) Sheep Polyclonal Antibody

CAT#: AP05063PU-N

VAV2 (SHD Region) sheep polyclonal antibody, Purified

Product Images

HEK293 cells were transfected with either emply expression vector (pSRa) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 µl/300 ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 µl/300 ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 µl/300 ug lysate). DPH or SHD anti Vav2 antisera (2.5 µl/300 ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: Western Blot: 1:5,000.
  Immunoprecipitation.
• Reactivities: Human
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A his tagged fusion protein Vav-2 corresponding to amino acid sequence
• Specificity: This antibody reacts to Vav-2 SHD.
• Formulation: Phosphate buffered saline with 0.08% sodium azide
  State: Purified
  State: Liquid purified Ig (0.2 µm sterile filtered)
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch
• Gene Name: vav guanine nucleotide exchange factor 2
• Database Link:
  Entrez Gene 7410 Human
  UniProt ID P52735
• Background: The immunogen used for the anti-human Vav-2 SHD region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 578 - 878 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50% homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl- homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
• Synonyms: VAV-2