MAGE 1 (MAGEA1) Mouse Monoclonal Antibody [Clone ID: MA454]

CAT#: AM32863PU-T

MAGE 1 (MAGEA1) mouse monoclonal antibody, clone MA454, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human testis stained with MAGE-1 Antibody (Clone MA454).

Specifications

Product Data

• Clone Name: MA454
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA (Use Antibody without BSA For coating).
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formallin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas.
• Reactivities: Canine, Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human MAGE-A1 full length recombinant protein.
• Specificity: This Monoclonal MA454 antibody recognizes a protein of 42-46kDa, identified as MAGE-1 and does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42-46 kDa
• Gene Name: MAGE family member A1
• Database Link:
  Entrez Gene 4100 Human
  UniProt ID P43355
• Background: MAGE-1 is a cancer testis antigen (CTA) expressing in a variety of human cancers (Pereira CM et al., 2012). It is a human melanoma antigen recognized by cytolytic T lymphocytes. MAGE-1 induces a tumor specific immune response so it is thought to be a potential therapeutic target for cancer immunotherapy (Ma ZY et al., 2002). The gene for MAGE-1 belongs to a family of 12 genes known as MAGE-A cluster located on the X chromosome in region q28 (Laduron S et al., 2004). This relies primarily on DNA methylation for repression in somatic tissues but in many types of tumors, the promoter of these genes becomes demethylated and transcription becomes activated (Loriot A et al., 2006). Monoclonal antibody aganist a recombinant feline melanoma antigen 1 (fMAGE-1) has been generated and is effective against feline lymphoma cell lines and tumor tissues (Ma ZY et al., 2002).
• Synonyms: MAGE 1, MAGE1, Melanoma-associated antigen 1, MAGEA1, MAGE1A