Mbl1 Rat Monoclonal Antibody [Clone ID: 8G6]

CAT#: AM26335PU-N

Mbl1 rat monoclonal antibody, clone 8G6, Purified

Product Images

MBL-A (8G6) deposition in developing murine atherosclerotic lesions. Staining of frozen tissue sections with antibody 8G6. Anti-mouse MBL-A at 2ug/ml (2h, RT). MBL-A was detected on the intima to media border as well as throughout the media (insert). Furthermore, extensive MBL-A deposition was seen at sites of necrosis (upper right corner).

Specifications

Product Data

• Clone Name: 8G6
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections (2,6): Tissue sections were fixed in 4% PBS-buffered formaldehyde and pretreated with 2% hydrogen peroxide in methanol for 20 minutes at 4⁰C to quench endogenous peroxidases. Primary antibody 8g6, 2µg/ml. As negative control rat IgG2a was used (Ref.2). The typical starting working dilution is 1:50.
  Immunoassays (2).
  Immunoflourescence (3,4).
  Western blot (1): A non-reduced sample treatment was used. The band sizes are 191, 263 and 316 kDa (Ref.1). The typical starting working dilution is 1:50.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Purified mouse MBL-A
• Specificity: This antibody detects MBL-A.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein G
• Conjugation: Unconjugated
• Gene Name: mannose-binding lectin (protein A) 1
• Database Link:
  Entrez Gene 17194 Mouse
  UniProt ID P39039
• Background:

  Mannose Binding Lectin (MBL), also called mannosebinding protein (MBP), is a calcium dependent oligomeric protein that belongs to the collectin family of proteins. It contains a collagen-like domain and a carbohydrate recognition domain enabling MBL to recognize carbohydrates (such as mannose and N-acetylglucosamine) on pathogens. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways, by using attached mannose binding lectin-associated serine proteases (MASP-2) in an antibody- and C1q-independent manner. MASP-2 permits cleavage of C4 and C2 to form a C3 convertase. Once it has bound, MBL exhibits complement-dependent antibacterial activities such as microbial opsonization and/or microbial lysis via membrane attack complexes and therefore plays an important role in innate immunity.
  In human, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, termed MBL-A and MBL-C. The MBL-A concentration in serum is about 6-fold lower compared to that of MBL-C... MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. Moreover, it has been shown that MBL-A deficient mice have aberrant antigen-specific IgM responses and suffer from increased susceptibility to infection.

• Synonyms: MBL-A; MBP-A; Mbpa; Mlb1; S-MBP
• Note: Note that the monoclonal antibody 8G6 is a calcium-dependent antibody.