MEKK2 (MAP3K2) Mouse Monoclonal Antibody [Clone ID: 4B4]

CAT#: AM06683SU-N

MEKK2 (MAP3K2) mouse monoclonal antibody, clone 4B4, Ascites

Product Images

Western blot analysis using MAP3K2 mAb against human MAP3K2 (AA: 148-359) recombinant protein. (Expected MW is 49.2 kDa)

• 

  Western blot analysis using MAP3K2 mAb against HEK293 (1) and MAP3K2 (AA: 148-359)--hIgGFc transfected HEK293 (2) cell lysate.

• 

  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

• 

  Immunofluorescence analysis of 3T3-L1 cells using MAP3K2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Product Data

• Clone Name: 4B4
• Applications: ELISA, IF, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human MAP3K2 expressed in E. Coli.
  
• Specificity: This antibody reacts to MAP3K2.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 70 kDa
• Gene Name: mitogen-activated protein kinase kinase kinase 2
• Database Link:
  Entrez Gene 10746 Human
  UniProt ID Q9Y2U5
• Background: The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.
• Synonyms: MAPKKK2, MEKK2