B7-2 (CD86) Mouse Monoclonal Antibody [Clone ID: 1B3]

CAT#: AM06519SU-N

B7-2 (CD86) mouse monoclonal antibody, clone 1B3, Ascites

Product Images

Western blot analysis using CD86 antibody Cat.-No AM06519SU-N against L1210 (1) and MOLT-4 (2) cell lysate.

Specifications

Product Data

• Clone Name: 1B3
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CD86 expressed in E. Coli.
• Specificity: Recognizes CD86
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 52 kDa
• Gene Name: CD86 molecule
• Database Link:
  Entrez Gene 942 Human
  UniProt ID P42081
• Background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.Tissue specificity: Expressed by activated B-lymphocytes and monocytes.
• Synonyms: CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1