CDC25C Mouse Monoclonal Antibody [Clone ID: 1F12]

CAT#: AM06420SU-N

CDC25C mouse monoclonal antibody, clone 1F12, Ascites

Product Images

Western blot analysis using anti-CDC25C antibody Cat.-No AM06420SU-N against Hela (Lane 1), K562 (Lane 2), PC-3 (Lane 3), HEK293 (Lane 4) and Raw264.7 (Lane 5) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human Testis tissues using anti-CDC25C antibody Cat.-No AM06420SU-N

Specifications

Product Data

• Clone Name: 1F12
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western blot: 1/500-1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CDC25C expressed in E. Coli.
• Specificity: Recognizes Protein Tyrosine Phosphatase CDC25C.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cell division cycle 25C
• Database Link:
  Entrez Gene 995 Human
  UniProt ID P30307
• Background:
  Cyclin-dependent kinases can be activated by CDC25, which removes inhibitory phosphates from tyrosine and threonine residues. At least three CDC25 genes (CDC25A, CDC25B, and CDC25C) have been identified in humans. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
  
  Cdc25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It has been highly conserved during evolution and it plays a key role in the regulation of cell division. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Cdc25C is mainly expressed in G2 phase. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
• Synonyms: CDC25