PYK2 (PTK2B) pTyr402 Mouse Monoclonal Antibody [Clone ID: 14F6]

CAT#: AM00130PU-N

PYK2 (PTK2B) pTyr402 mouse monoclonal antibody, clone 14F6, Purified

Product Images

Transactivation of PYK2 Whole cell lysates of serum starved HepG2 tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab PYK2-14F6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1:Co; lane 2: serum; lane 3: EGF; lane 4: H2O2;lane 5: Anisomycin; lane 6: Sorbit; lane 7: Arsen; lane 8:Ceramide.

Specifications

Product Data

• Clone Name: 14F6
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.05 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH
• Specificity: This antibody specifically interacts with activated Pyk2/CAKβ phosphorylated at tyrosine 402. The antibody does not crossreact with the non-phosphorylated form of Pyk2/CAKβ nor with unrelated phosphorylation sites.
• Formulation: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein tyrosine kinase 2 beta
• Database Link:
  Entrez Gene 2185 Human
  UniProt ID Q14289
• Background: Proline-rich tyrosine kinase 2 (Pyk2; also known as cellular adhesion kinase β, CAK β) is a 116 kDa cytoplasmic tyrosine kinase. Pyk2 is rapidely phosphorylated on tyrosine residues in response to activation by G-protein-coupled receptors as well as stress signals. Phosphorylation of Pyk2 leads to activation of stress-activated kinase 1 and 2 (SAPK1 and SAPK2).
• Synonyms: PYK2, RAFTK, Protein tyrosine kinase 2 beta, Focal adhesion kinase 2, CAK-beta, CADTK
• Note: Molecular Weight: 116kDa
  Epitope: phosphotyrosine 402 S D I pY A E I