NrCAM (NM_005010) Human Untagged Clone

CAT#: SC108536

NRCAM (untagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NRCAM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005010, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGCTTAAAATAATGCCGAAAAAGAAGCGCTTATCTGCGGGCAGAGTGCCCCTGATTCTCTTCCTGT
  GCCAGATGATTAGTGCACTGGAAGTACCTCTTGATCTGGTACAGCCTCCAACCATCACCCAACAGTCTCC
  AAAAGATTACATTATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAAGCCAAAGGGAAACCGCCCCCA
  AGCTTTTCCTGGACCCGTAATGGGACTCATTTTGACATCGATAAAGACCCTCTGGTCACCATGAAGCCTG
  GCACAGGAACGCTCATAATTAACATCATGAGCGAAGGGAAAGCTGAGACCTATGAAGGAGTCTATCAGTG
  TACAGCAAGGAACGAACGCGGAGCTGCAGTTTCTAATAACATTGTTGTCCGCCCATCCAGATCACCATTG
  TGGACCAAAGAAAAACTTGAACCAATCACACTTCAAAGTGGTCAGTCTTTAGTACTTCCCTGCAGACCCC
  CAATTGGATTACCACCACCTATAATATTTTGGATGGATAATTCCTTTCAAAGACTTCCACAAAGTGAGAG
  AGTTTCTCAAGGTTTGAATGGGGACCTTTATTTTTCCAATGTCCTCCCAGAGGACACCCGCGAAGACTAT
  ATCTGTTATGCTAGATTTAATCATACTCAAACCATACAGCAGAAGCAACCTATTTCTGTGAAGGTGATTT
  CAGTGGATGAATTGAATGACACTATAGCTGCTAATTTGAGTGACACTGAGTTTTATGGTGCTAAATCAAG
  TAGAGAGAGGCCACCAACATTTTTAACTCCAGAAGGCAATGCAAGTAACAAAGAGGAATTAAGAGGAAAT
  GTGCTTTCACTGGAGTGCATTGCAGAAGGACTGCCTACCCCAATTATTTACTGGGCAAAGGAAGATGGAA
  TGCTACCCAAAAACAGGACAGTTTATAAGAACTTTGAGAAAACCTTGCAGATCATTCATGTTTCAGAAGC
  AGACTCTGGAAATTACCAATGTATAGCAAAAAACGCATTAGGAGCCATCCACCATACCATTTCTGTTAGA
  GTTAAAGCGGCTCCATACTGGATCACAGCCCCTCAAAATCTTGTGCTGTCCCCAGGAGAGGATGGGACCT
  TGATCTGCAGAGCTAATGGCAACCCCAAACCCAGAATTAGCTGGTTAACAAATGGAGTCCCAATAGAAAT
  TGCCCCTGATGACCCCAGCAGAAAAATAGATGGCGATACCATTATTTTTTCAAATGTTCAAGAAAGATCA
  AGTGCAGTCTATCAGTGCAATGCCTCTAATGAATATGGATATTTACTGGCAAACGCATTTGTAAATGTGC
  TGGCTGAGCCACCACGAATCCTCACACCTGCAAACACACTCTACCAGGTCATTGCAAACAGGCCTGCTTT
  ACTAGACTGTGCCTTCTTTGGGTCTCCTCTCCCAACCATCGAGTGGTTTAAAGGAGCTAAAGGAAGTGCT
  CTTCATGAAGATATTTATGTTTTACATGAAAATGGAACTTTGGAAATTCCTGTGGCCCAAAAGGACAGTA
  CAGGAACTTATACGTGTGTTGCAAGGAATAAATTAGGGATGGCGAAGAATGAAGTTCACTTAGAAATCAA
  AGATCCTACATGGATCGTTAAACAGCCCGAATATGCAGTTGTGCAAAGAGGGAGCATGGTGTCCTTTGAA
  TGCAAAGTGAAACATGATCACACCTTATCCCTCACTGTCCTGTGGCTGAAGGACAACAGGGAACTGCCCA
  GTGATGAAAGGTTCACTGTTGACAAGGATCATCTAGTGGTAGCTGATGTCAGTGACGATGACAGCGGGAC
  CTACACGTGTGTGGCCAACACCACTCTGGACAGCGTCTCCGCCAGCGCTGTGCTTAGCGTTGTTGATGTC
  CCAAATCCTCCCTTTGACTTAGAACTGACAGATCAACTTGACAAAAGTGTTCAGCTGTCATGGACCCCAG
  GCGATGACAACAATAGCCCCATTACAAAATTCATCATCGAATATGAAGATGCAATGCACAAGCCAGGGCT
  GTGGCACCACCAAACTGAAGTTTCTGGAACACAGACCACAGCCCAGCTGAAGCTGTCTCCTTACGTGAAC
  TACTCCTTCCGCGTGATGGCAGTGAACAGCATTGGGAAGAGCTTGCCCAGCGAGGCGTCTGAGCAGTATT
  TGACGAAAGCCTCAGAACCAGATAAAAACCCCACAGCTGTGGAAGGACTGGGATCAGAGCCTGATAATTT
  GGTGATTACGTGGAAGCCCTTGAATGGTTTCGAATCTAATGGGCCAGGCCTTCAGTACAAAGTTAGCTGG
  CGCCAGAAAGATGGTGATGATGAATGGACATCTGTGGTTGTGGCAAATGTATCCAAATATATTGTCTCAG
  GCACGCCAACCTTTGTTCCATACCTGATCAAAGTTCAGGCCCTGAATGACATGGGGTTTGCCCCCGAGCC
  AGCTGTAGTCATGGGACATTCTGGAGAAGACCTCCCAATGGTGGCTCCTGGGAACGTGCGTGTGAATGTG
  GTGAACAGTACCTTAGCCGAGGTGCACTGGGACCCAGTACCTCTGAAAAGCATCCGAGGACACCTACAAG
  GCTATCGGATTTACTATTGGAAGACCCAGAGTTCATCTAAAAGAAACAGACGTCACATTGAGAAAAAGAT
  CCTCACCTTCCAAGGCAGCAAGACTCATGGCATGTTGCCGGGGCTAGAGCCCTTTAGCCACTACACACTG
  AATGTCCGAGTGGTCAATGGGAAAGGGGAGGGCCCAGCCAGCCCTGACAGAGTCTTTAATACTCCAGAAG
  GAGTCCCCAGTGCTCCCTCGTCTTTGAAGATTGTGAATCCAACACTGGACTCTCTCACTTTGGAATGGGA
  TCCACCGAGCCACCCGAATGGCATTTTGACAGAGTACACCTTAAAGTATCAGCCAATTAACAGCACACAT
  GAATTAGGCCCTCTGGTAGATTTGAAAATTCCTGCCAACAAGACACGGTGGACTTTAAAAAATTTAAATT
  TCAGCACTCGATATAAGTTTTATTTCTATGCACAAACATCAGCAGGATCAGGAAGTCAAATTACAGAGGA
  AGCAGTAACAACTGTGGATGAAGCGATGGCAAGCCGGCAGGTGGATATTGCAACTCAGGGCTGGTTCATT
  GGTCTGATGTGTGCTGTTGCTCTCCTTATCTTAATTTTGCTGATTGTTTGCTTCATCAGAAGAAACAAGG
  GTGGTAAATATCCAGTTAAAGAAAAGGAAGATGCCCATGCTGACCCTGAAATCCAGCCTATGAAGGAAGA
  TGATGGGACATTTGGAGAATACAGTGATGCAGAAGACCACAAGCCTTTGAAAAAAGGAAGTCGAACTCCT
  TCAGACAGGACTGTGAAAAAAGAAGATAGTGACGACAGCCTAGTTGACTATGGAGAAGGGGTTAATGGCC
  AGTTCAATGAGGATGGCTCCTTTATTGGACAATACAGTGGTAAGAAAGAGAAAGAGCCGGCTGAAGGAAA
  CGAAAGCTCAGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTTGTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005010 unedited
  NGGGTAAATTTTTGATATTTGTATACGACTTACTATAGGCGGCCGCGCAATTCGCACGAG
  GCTTCGTCGAGAACGGAGGACACCGGCGGTCCAGGGTCCTGGGCAGTCGCGCCAGAGCTG
  AGCGGAGGGCGCGGCGCGAGAACGAATCTTTGTGACATTCTCTCTCAGCATTCTTTATCC
  CCTGTTTGCTGAAGACTTCGACAAAAGCTGGTCTTAGCTGTTGGCATTCTCCTGAGAAAA
  GGATAGCTTCAGAAATCAGAAAAACATTTGGGAGGTGTCTAGCCCAGTGGACCTTCTGAA
  GAGCAATGCTAAGAAGACGTTTGGTTTAAAGAATTAAAAGGAAGAACAACTTAAGAGCTT
  CTTCAAAGTTTCCCGCATGAAAATTACTTAAACGTTGCACACAACGTTTCACAAAATCTT
  TTGTGAAAGAAGAAAAGGAATTCAGTGTGTGAGTCTCAGCAGGAGTTAAGCTAATGCAGC
  TTAAAATAATGGCCGAANAAGAAGCGCTTATCTGGCGGCAGAGTGCCCCTGATTCTCTTC
  CTGTGCCAGATGATTAGTGCACTGGAAGTACCTCTTGATCCAAAACTTCCTGAAGACTTG
  GTACAGGCTTCAACCATTACCCAACAGTCTTCAAAGAATACATTATTGACCCTCGGGAGA
  ATATTGGGATCCAGTGTGAAGCCCAGGGAAACCGGCCCCAAGCTTTTCCTGGGCCCGTAT
  GGGACTCAATTTGACATCGATAAGACCCTCTGGTGACATGAAACTGGGGCACGAACGCTC
  TTATTTACATCTGGGCCAAAGGGAAGCTTAGACCCTTGAAGGTCTTTCACGTACCACAAG
  GACCAACCCGCACTGCCGTTCTTATACACTGTGTGCCCCCTTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005010 unedited
  ACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTAACTTTTAAAACCACATTTAATTTTTAATACCAAATCTGTTGCACTATTACAAAAGT
  GACCCTTTCATGCACAGATAAATTTCGCATTTACCAGTATGGAAAAGGAAAAAATGTAGT
  TTTATTGTTCTCCCAAAATATTGGCAAATTTAAAGTAACTTTTAATCTTTGGGCTCTCTG
  ATGCCAAGGCAAACCTGTTTTGTCTTTTTATATTTTCAAAAAACAGGGGGATCTGTGAAA
  AATGAATTTAGAGACCTGAAAGTTTTTAGGGCCATCTTTACCTGGGATTACTTTAAATTA
  TGCTTTACCTACATATATCAAACATGCCAGGTTACTAGGCCTACTGTGCCCCATAGGCAA
  AGCTCTGAAGATTTCATCGAAAAATCTGCTGTCAATACGTAAAAAAGTTCACTATTTCAG
  TTTCACAGCAAAAAAGGTGGGGGGAGGGGGGAACCCAATAGATATTTAAGTAGATGCTTT
  CCAATCCCATTCACTGCATTAATTAGCTTACCTCTTATACAGTACAACATAAACATTGCA
  TGTTTATTTGTATGTAACACCTATAAGCATATAGCATCTACATTTTAAGTGTATTTACAA
  ATTCAACAAAATATCTACATATAAAAAGCTTTACTTAAAATTAAACTTGATGCAAGTTAT
  GAGAAACCAATTTATTGGCAAATGAAACTGAGCATTCCTTCAACCATAGGTTGTTATAGA
  TTTTCATATTTTGAAGGGGACCCCATTGATAGATTTGGTTATGATAACCATNGATATATA
  TTACTTTTTAAACTGCAAATGGTATCCCTTGGACCGGTGGCCTCTTTAGCACTTGCCTGA
  TTCTTAAATTCTGAAAGGATCCCATACAATTTGCATCACCGTGTTAAGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005010
• Insert Size: 6450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005010.2, NP_005001.2
• RefSeq Size: 6322 bp
• RefSeq ORF: 3552 bp
• Locus ID: 4897
• UniProt ID: Q92823
• Cytogenetics: 7q31.1
• Domains: ig, IGc2, IG, FN3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks five in-frame exons in the coding region, compared to variant 1. The encoded protein (isoform B) is shorter than isoform A.