NrCAM (NM_005010) Human Tagged ORF Clone

CAT#: RG214900

NRCAM (tGFP-tagged) - Human neuronal cell adhesion molecule (NRCAM), transcript variant 2

Product Images

Circular map for RG214900

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NRCAM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214900 representing NM_005010
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCTTAAAATAATGCCGAAAAAGAAGCGCTTATCTGCGGGCAGAGTGCCCCTGATTCTCTTCCTGT
  GCCAGATGATTAGTGCACTGGAAGTACCTCTTGATCTGGTACAGCCTCCAACCATCACCCAACAGTCTCC
  AAAAGATTACATTATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAAGCCAAAGGGAAACCGCCCCCA
  AGCTTTTCCTGGACCCGTAATGGGACTCATTTTGACATCGATAAAGACCCTCTGGTCACCATGAAGCCTG
  GCACAGGAACGCTCATAATTAACATCATGAGCGAAGGGAAAGCTGAGACCTATGAAGGAGTCTATCAGTG
  TACAGCAAGGAACGAACGCGGAGCTGCAGTTTCTAATAACATTGTTGTCCGCCCATCCAGATCACCATTG
  TGGACCAAAGAAAAACTTGAACCAATCACACTTCAAAGTGGTCAGTCTTTAGTACTTCCCTGCAGACCCC
  CAATTGGATTACCACCACCTATAATATTTTGGATGGATAATTCCTTTCAAAGACTTCCACAAAGTGAGAG
  AGTTTCTCAAGGTTTGAATGGGGACCTTTATTTTTCCAATGTCCTCCCAGAGGACACCCGCGAAGACTAT
  ATCTGTTATGCTAGATTTAATCATACTCAAACCATACAGCAGAAGCAACCTATTTCTGTGAAGGTGATTT
  CAGTGGATGAATTGAATGACACTATAGCTGCTAATTTGAGTGACACTGAGTTTTATGGTGCTAAATCAAG
  TAGAGAGAGGCCACCAACATTTTTAACTCCAGAAGGCAATGCAAGTAACAAAGAGGAATTAAGAGGAAAT
  GTGCTTTCACTGGAGTGCATTGCAGAAGGACTGCCTACCCCAATTATTTACTGGGCAAAGGAAGATGGAA
  TGCTACCCAAAAACAGGACAGTTTATAAGAACTTTGAGAAAACCTTGCAGATCATTCATGTTTCAGAAGC
  AGACTCTGGAAATTACCAATGTATAGCAAAAAACGCATTAGGAGCCATCCACCATACCATTTCTGTTAGA
  GTTAAAGCGGCTCCATACTGGATCACAGCCCCTCAAAATCTTGTGCTGTCCCCAGGAGAGGATGGGACCT
  TGATCTGCAGAGCTAATGGCAACCCCAAACCCAGAATTAGCTGGTTAACAAATGGAGTCCCAATAGAAAT
  TGCCCCTGATGACCCCAGCAGAAAAATAGATGGCGATACCATTATTTTTTCAAATGTTCAAGAAAGATCA
  AGTGCAGTCTATCAGTGCAATGCCTCTAATGAATATGGATATTTACTGGCAAACGCATTTGTAAATGTGC
  TGGCTGAGCCACCACGAATCCTCACACCTGCAAACACACTCTACCAGGTCATTGCAAACAGGCCTGCTTT
  ACTAGACTGTGCCTTCTTTGGGTCTCCTCTCCCAACCATCGAGTGGTTTAAAGGAGCTAAAGGAAGTGCT
  CTTCATGAAGATATTTATGTTTTACATGAAAATGGAACTTTGGAAATTCCTGTGGCCCAAAAGGACAGTA
  CAGGAACTTATACGTGTGTTGCAAGGAATAAATTAGGGATGGCGAAGAATGAAGTTCACTTAGAAATCAA
  AGATCCTACATGGATCGTTAAACAGCCCGAATATGCAGTTGTGCAAAGAGGGAGCATGGTGTCCTTTGAA
  TGCAAAGTGAAACATGATCACACCTTATCCCTCACTGTCCTGTGGCTGAAGGACAACAGGGAACTGCCCA
  GTGATGAAAGGTTCACTGTTGACAAGGATCATCTAGTGGTAGCTGATGTCAGTGACGATGACAGCGGGAC
  CTACACGTGTGTGGCCAACACCACTCTGGACAGCGTCTCCGCCAGCGCTGTGCTTAGCGTTGTTGATGTC
  CCAAATCCTCCCTTTGACTTAGAACTGACAGATCAACTTGACAAAAGTGTTCAGCTGTCATGGACCCCAG
  GCGATGACAACAATAGCCCCATTACAAAATTCATCATCGAATATGAAGATGCAATGCACAAGCCAGGGCT
  GTGGCACCACCAAACTGAAGTTTCTGGAACACAGACCACAGCCCAGCTGAAGCTGTCTCCTTACGTGAAC
  TACTCCTTCCGCGTGATGGCAGTGAACAGCATTGGGAAGAGCTTGCCCAGCGAGGCGTCTGAGCAGTATT
  TGACGAAAGCCTCAGAACCAGATAAAAACCCCACAGCTGTGGAAGGACTGGGATCAGAGCCTGATAATTT
  GGTGATTACGTGGAAGCCCTTGAATGGTTTCGAATCTAATGGGCCAGGCCTTCAGTACAAAGTTAGCTGG
  CGCCAGAAAGATGGTGATGATGAATGGACATCTGTGGTTGTGGCAAATGTATCCAAATATATTGTCTCAG
  GCACGCCAACCTTTGTTCCATACCTGATCAAAGTTCAGGCCCTGAATGACATGGGGTTTGCCCCCGAGCC
  AGCTGTAGTCATGGGACATTCTGGAGAAGACCTCCCAATGGTGGCTCCTGGGAACGTGCGTGTGAATGTG
  GTGAACAGTACCTTAGCCGAGGTGCACTGGGACCCAGTACCTCTGAAAAGCATCCGAGGACACCTACAAG
  GCTATCGGATTTACTATTGGAAGACCCAGAGTTCATCTAAAAGAAACAGACGTCACATTGAGAAAAAGAT
  CCTCACCTTCCAAGGCAGCAAGACTCATGGCATGTTGCCGGGGCTAGAGCCCTTTAGCCACTACACACTG
  AATGTCCGAGTGGTCAATGGGAAAGGGGAGGGCCCAGCCAGCCCTGACAGAGTCTTTAATACTCCAGAAG
  GAGTCCCCAGTGCTCCCTCGTCTTTGAAGATTGTGAATCCAACACTGGACTCTCTCACTTTGGAATGGGA
  TCCACCGAGCCACCCGAATGGCATTTTGACAGAGTACACCTTAAAGTATCAGCCAATTAACAGCACACAT
  GAATTAGGCCCTCTGGTAGATTTGAAAATTCCTGCCAACAAGACACGGTGGACTTTAAAAAATTTAAATT
  TCAGCACTCGATATAAGTTTTATTTCTATGCACAAACATCAGCAGGATCAGGAAGTCAAATTACAGAGGA
  AGCAGTAACAACTGTGGATGAAGCGATGGCAAGCCGGCAGGTGGATATTGCAACTCAGGGCTGGTTCATT
  GGTCTGATGTGTGCTGTTGCTCTCCTTATCTTAATTTTGCTGATTGTTTGCTTCATCAGAAGAAACAAGG
  GTGGTAAATATCCAGTTAAAGAAAAGGAAGATGCCCATGCTGACCCTGAAATCCAGCCTATGAAGGAAGA
  TGATGGGACATTTGGAGAATACAGTGATGCAGAAGACCACAAGCCTTTGAAAAAAGGAAGTCGAACTCCT
  TCAGACAGGACTGTGAAAAAAGAAGATAGTGACGACAGCCTAGTTGACTATGGAGAAGGGGTTAATGGCC
  AGTTCAATGAGGATGGCTCCTTTATTGGACAATACAGTGGTAAGAAAGAGAAAGAGCCGGCTGAAGGAAA
  CGAAAGCTCAGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214900 representing NM_005010
  Red=Cloning site Green=Tags(s)
  
  MQLKIMPKKKRLSAGRVPLILFLCQMISALEVPLDLVQPPTITQQSPKDYIIDPRENIVIQCEAKGKPPP
  SFSWTRNGTHFDIDKDPLVTMKPGTGTLIINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVRPSRSPL
  WTKEKLEPITLQSGQSLVLPCRPPIGLPPPIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPEDTREDY
  ICYARFNHTQTIQQKQPISVKVISVDELNDTIAANLSDTEFYGAKSSRERPPTFLTPEGNASNKEELRGN
  VLSLECIAEGLPTPIIYWAKEDGMLPKNRTVYKNFEKTLQIIHVSEADSGNYQCIAKNALGAIHHTISVR
  VKAAPYWITAPQNLVLSPGEDGTLICRANGNPKPRISWLTNGVPIEIAPDDPSRKIDGDTIIFSNVQERS
  SAVYQCNASNEYGYLLANAFVNVLAEPPRILTPANTLYQVIANRPALLDCAFFGSPLPTIEWFKGAKGSA
  LHEDIYVLHENGTLEIPVAQKDSTGTYTCVARNKLGMAKNEVHLEIKDPTWIVKQPEYAVVQRGSMVSFE
  CKVKHDHTLSLTVLWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVANTTLDSVSASAVLSVVDV
  PNPPFDLELTDQLDKSVQLSWTPGDDNNSPITKFIIEYEDAMHKPGLWHHQTEVSGTQTTAQLKLSPYVN
  YSFRVMAVNSIGKSLPSEASEQYLTKASEPDKNPTAVEGLGSEPDNLVITWKPLNGFESNGPGLQYKVSW
  RQKDGDDEWTSVVVANVSKYIVSGTPTFVPYLIKVQALNDMGFAPEPAVVMGHSGEDLPMVAPGNVRVNV
  VNSTLAEVHWDPVPLKSIRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGSKTHGMLPGLEPFSHYTL
  NVRVVNGKGEGPASPDRVFNTPEGVPSAPSSLKIVNPTLDSLTLEWDPPSHPNGILTEYTLKYQPINSTH
  ELGPLVDLKIPANKTRWTLKNLNFSTRYKFYFYAQTSAGSGSQITEEAVTTVDEAMASRQVDIATQGWFI
  GLMCAVALLILILLIVCFIRRNKGGKYPVKEKEDAHADPEIQPMKEDDGTFGEYSDAEDHKPLKKGSRTP
  SDRTVKKEDSDDSLVDYGEGVNGQFNEDGSFIGQYSGKKEKEPAEGNESSEAPSPVNAMNSFV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005010
• ORF Size: 3549 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005010.4, NP_005001.3
• RefSeq Size: 6322 bp
• RefSeq ORF: 3552 bp
• Locus ID: 4897
• UniProt ID: Q92823
• Cytogenetics: 7q31.1
• Domains: ig, IGc2, IG, FN3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]