Cytochrome P450 17A1 (CYP17A1) (NM_000102) Human Untagged Clone

CAT#: SC102224

CYP17A1 (untagged)-Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome P450 17A1
• Synonyms: CPT7; CYP17; P450C17; S17AH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000102 edited
  ATAGGGCGGCCGCGAATTCGGCACGAGACTGCTGTCTATCTTGCCTGCCGGCACCCAGCC
  ACCATGTGGGAGCTCGTGGCTCTCTTGCTGCTTACCCTAGCTTATTTGTTTTGGCCCAAG
  AGAAGGTGCCCTGGTGCCAAGTACCCCAAGAGCCTCCTGTCCCTGCCCCTGGTGGGCAGC
  CTGCCATTCCTCCCCAGACACGGCCATATGCATAACAACTTCTTCAAGCTGCAGAAAAAA
  TATGGCCCCATCTATTCGGTTCGTATGGGCACCAAGACTACAGTGATTGTCGGCCACCAC
  CAGCTGGCCAAGGAGGTGCTTATTAAGAAGGGCAAGGACTTCTCTGGGCGGCCTCAAATG
  GCAACTCTAGACATCGCGTCCAACAACCGTAAGGGTATCGCCTTCGCTGACTCTGGCGCA
  CACTGGCAGCTGCATCGAAGGCTGGCGATGGCCACCTTTGCCCTGTTCAAGGATGGCGAT
  CAGAAGCTGGAGAAGATCATTTGTCAGGAAATCAGTACATTGTGTGATATGCTGGCCACC
  CACAACGGACAGTCCATAGACATCTCCTTTCCTGTCTTCGTGGCGGTAACCAATGTCATC
  TCCTTGATCTGCTTCAATACCTCCTACAAGAATGGGGACCCTGAGTTGAATGTCATACAG
  AATTACAATGAAGGCATCATAGACAACCTGAGCAAAGACAGCCTGGTGGACCTAGTCCCC
  TGGTTGAAGATTTTCCCCAACAAAACCCTGGAAAAATTAAAGAGCCATGTTAAAATACGA
  AATGATCTGCTGAATAAAATACTTGAAAATTACAAGGAGAAATTCCGGAGTGACTCTATC
  ACCAACATGCTGGACACACTGATGCAAGCCAAGATGAACTCAGATAATGGCAATGCTGGC
  CCAGATCAAGACTCAGAGCTGCTTTCAGATAACCACATTCTCACCACCATAGGGGACATC
  TTTGGGGCTGGCGTGGAGACCACCACCTCTGTGGTTAAATGGACCCTGGCCTTCCTGCTG
  CACAATCCTCAGGTGAAGAAGAAGCTCTACGAGGAGATTGACCAGAATGTGGGTTTCAGC
  CGCACACCAACTATCAGTGACCGTAACCGTCTCCTCCTGCTGGAGGCCACCATCCGAGAG
  GTGCTTCGCCTCAGGCCCGTGGCCCCTATGCTCATCCCCCACAAGGCCAACGTTGACTCC
  AGCATCGGTGAGTTTGCTGTGGACAAGGGCACAGAAGTTATCATCAATCTGTGGGCGCTG
  CATCACAATGAGAAGGAGTGGCACCAGCCGGATCAGTTCATGCCTGAGCGTTTCTTGAAT
  CCAGCGGGGACCCAGCTCATCTCACCGTCAGTAAGCTATTTGCCCTTCGGAGCAGGACCT
  CGCTCCTGTATAGGTGAGATCCTGGCCCGCCAGGAGCTCTTCCTCATCATGGCCTGGCTG
  CTGCAGAGGTTCGACCTGGAGGTGCCAGATGATGGGCAGCTGCCCTCCCTGGAAGGCATC
  CCCAAGGTGGTCTTTCTGATCGACTCTTTCAAAGTGAAGATCAAGGTGCGCCAGGCCTGG
  AGGGAAGCCCAGGCTGAGGGTAGCACCTAAAGGCTGTAACTCACAGCCCCTGTCCACCCT
  ATGTGGCCCCACAACACAGATTTAGAGATACAACCCCCCACCCTTCTCCGCCATTCTTCC
  CTACTCCCAACCCACTCTGCCTTCTTTTTCAGCTTGTGGCAATGCCAGTGATGTGCATAA
  ACAGTTTTTTTTTTTCCAAAAAAAAGTGAAAGGCAGCCTGGGAATGAACATTGTTAGTTC
  TATCTTGGCCTTCATTGGAGTGATTCTGCTGCTGGTGGATATGTGCATCAATGGGGTAGC
  TG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000102 unedited
  ATTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGACTGCTGTCT
  ATCTTGCCTGCCGGCACCCAGCCACCATGTGGGAGCTCGTGGCTCTCTTGCTGCTTACCC
  TAGCTTATTTGTTTTGGCCCAAGAGAAGGTGCCCTGGTGCCAAGTACCCCAAGAGCCTCC
  TGTCCCTGCCCCTGGTGGGCAGCCTGCCATTCCTCCCCAGACACGGCCATATGCATAACA
  ACTTCTTCAAGCTGCAGAAAAAATATGGCCCCATCTATTCGGTTCGTATGGGCACCAAGA
  CTACAGTGATTGTCGGCCACCACCAGCTGGCCAAGGAGGTGCTTATTAAGAAGGGCAAGG
  ACTTCTCTGGGCGGCCTCAAATGGCAACTCTAGACATCGCGTCCAACAACCGTAAGGGTA
  TCGCCTTCGCTGACTCTGGCGCACACTGGCAGCTGCATCGAAGGCTGGCGATGGCCACCT
  TTGCCCTGTTCAAGGATGGCGATCAGAAGCTGGAGAAGATCATTTGTCAGGAAATCAGTA
  CATTGTGTGATATGCTGGCCACCCACAACGGACAGTCCATAGACATCTCCTTTCCTGTCT
  TCGTGGCGGTAACCAATGTCATCTCCTTGATCTGCTTCAATACCTCCTACAAGAATGGGG
  ACCCTGAGTTGAATGTCATACAGAATTACAATGAAGGCATCATAGACAACCTGAGCAAAG
  ACAGCCTGGTGGACCTAGTCCCNCTGTTGAAAGATTTCCCCACAAAACCCTGNAAAAATA
  AAGAGCCATGTAAAATACGAATGATCTGCTGAATAAAATACTGANAATACAGGAGAAANT
  NCCGATGACTCTTCACCACATGCTGGAACACTGATGCAGCCCAAGATGACTCANATATGG
  CATGCTGGCCAGATCAGATCAAACTGTTTCGATACCACATCTACACATAGGGACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000102 unedited
  CTATGGAACGGCGGCCGCAATCTAGGATCGAGTTTTNNNNNNTTTTTTTTTTTTTTTTTT
  TTTTTTTTTGGGNNCTAAGGACCTTTTATTAAGGAAAAAGTAATTTGAACAGAAACATTT
  TTAACTTTAAAAAAGTTGCAGAATCATTTTTCAGTCCTCTGATCCTTCACCTTTGCCAGC
  ATGTAATAGTGGTAATGTAGGAAAAAACAGTGAAGGGAGATACAGCCATTATTAGCGACC
  AACCTAAAAACAAATTTCAAACACAGTTTTTAAAGACTAGAAATCATTGTAGACAATAAA
  AGACATTTTTTTAAAAAATTTTTGCAAGGGGTTTTTCTCCATGAAATTAAACTGATACCC
  CTTTTTCTTTTACTATTTAGGGGCATTAACTGAGTAGTTGTTGCATCTGGGAGGAGCTGA
  AAAAAACCCTGGGGTCACAGGGTTGCTTTCATACATATTTGGAATAACCAGGACAGACAT
  ATTGGTTGGGGGGTTTGCTTGGTTGGCAAAATGGGCTGTGGCACAAGCTACGAAGAACTC
  CAAGAGGGAGAAGATCATCAGCGTGGCTGAAATGCCTTTTCCAGAAAGCACGGCCCAGTA
  GTCTTGGCCAGCTACCCCATTGATGCACATATCCACCAGCAGCAGAATCACTCCAATGAA
  GGCCAAGATAGAACTAACAATGTTCATTCCCAGGCTGGCTTTTACTTTTTTTTGGGAAAA
  AAAAACTGTTTTGCACATCACTGGCATTGCCACAAGCTGAAAAAAAAGGCANAATGGTTN
  GGGAGTAGGGAAAAATGGCGGGAAAAGGTGGGGGGGTTGTATCTCTAAATTGGTGTGTGG
  GGCCCCATAGGGTTGACAGGGGCTTGGGAGTACAGCCTTTAGGGCTACCCTCACCTGGGC
  TTNCCTCAGGCTGGCGCCCTTGACTNACTTTGAAGAACATCACAAAACCCCTGGGGAGCT
  TCCAGAAGGCACTGCCATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000102
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000102.2, NP_000093.1
• RefSeq Size: 1755 bp
• RefSeq ORF: 1527 bp
• Locus ID: 1586
• UniProt ID: P05093
• Cytogenetics: 10q24.32
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]