Cytochrome P450 17A1 (CYP17A1) (NM_000102) Human Tagged ORF Clone

CAT#: RC209042

CYP17A1 (Myc-DDK-tagged)-Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1)

Product Images

Circular map for RC209042

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP17A1 (Cat# RC209042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1(Cat# TA503442). Positive lysates LY400030 (100ug) and LC400030 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400030) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209042 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CYP17A1 protein (Cat# TP309042). The protein was produced from HEK293T cells transfected with CYP17A1 cDNA clone (Cat# RC209042) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cytochrome P450 17A1
• Synonyms: CPT7; CYP17; P450C17; S17AH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209042 representing NM_000102
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGAGCTCGTGGCTCTCTTGCTGCTTACCCTAGCTTATTTGTTTTGGCCCAAGAGAAGGTGCCCTG
  GTGCCAAGTACCCCAAGAGCCTCCTGTCCCTGCCCCTGGTGGGCAGCCTGCCATTCCTCCCCAGACATGG
  CCATATGCATAACAACTTCTTCAAGCTGCAGAAAAAATATGGCCCCATCTATTCTGTTCGTATGGGCACC
  AAGACTACAGTGATTGTCGGCCACCACCAGCTGGCCAAGGAGGTGCTTATTAAGAAGGGCAAGGACTTCT
  CTGGGCGGCCTCAAATGGCAACTCTAGACATCGCGTCCAACAACCGTAAGGGTATCGCCTTCGCTGACTC
  TGGCGCACACTGGCAGCTGCATCGAAGGCTGGCGATGGCCACCTTTGCCCTGTTCAAGGATGGCGATCAG
  AAGCTGGAGAAGATCATTTGTCAGGAAATCAGTACATTGTGTGATATGCTGGCCACCCACAACGGACAGT
  CCATAGACATCTCCTTTCCTGTCTTCGTGGCGGTAACCAATGTCATCTCCTTGATCTGCTTCAATACCTC
  CTACAAGAATGGGGACCCTGAGTTGAATGTCATACAGAATTACAATGAAGGCATCATAGACAACCTGAGC
  AAAGACAGCCTGGTGGACCTAGTCCCCTGGTTGAAGATTTTCCCCAACAAAACCCTGGAAAAATTAAAGA
  GCCATGTTAAAATACGAAATGATCTGCTGAATAAAATACTTGAAAATTACAAGGAGAAATTCCGGAGTGA
  CTCTATCACCAACATGCTGGACACACTGATGCAAGCCAAGATGAACTCAGATAATGGCAATGCTGGCCCA
  GATCAAGACTCAGAGCTGCTTTCAGATAACCACATTCTCACCACCATAGGGGACATCTTTGGGGCTGGCG
  TGGAGACCACCACCTCTGTGGTTAAATGGACCCTGGCCTTCCTGCTGCACAATCCTCAGGTGAAGAAGAA
  GCTCTACGAGGAGATTGACCAGAATGTGGGTTTCAGCCGCACACCAACTATCAGTGACCGTAACCGTCTC
  CTCCTGCTGGAGGCCACCATCCGAGAGGTGCTTCGCCTCAGGCCCGTGGCCCCTATGCTCATCCCCCACA
  AGGCCAACGTTGACTCCAGCATCGGTGAGTTTGCTGTGGACAAGGGCACAGAAGTTATCATCAATCTGTG
  GGCGCTGCATCACAATGAGAAGGAGTGGCACCAGCCGGATCAGTTCATGCCTGAGCGTTTCTTGAATCCA
  GCGGGGACCCAGCTCATCTCACCGTCAGTAAGCTATTTGCCCTTCGGAGCAGGACCTCGCTCCTGTATAG
  GTGAGATCCTGGCCCGCCAGGAGCTCTTCCTCATCATGGCCTGGCTGCTGCAGAGGTTCGACCTGGAAGT
  GCCAGATGATGGGCAGCTGCCCTCCCTGGAAGGCATCCCCAAGGTGGTCTTTCTGATCGACTCTTTCAAA
  GTGAAGATCAAGGTGCGCCAGGCCTGGAGGGAAGCCCAGGCTGAGGGTAGCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209042 representing NM_000102
  Red=Cloning site Green=Tags(s)
  
  MWELVALLLLTLAYLFWPKRRCPGAKYPKSLLSLPLVGSLPFLPRHGHMHNNFFKLQKKYGPIYSVRMGT
  KTTVIVGHHQLAKEVLIKKGKDFSGRPQMATLDIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQ
  KLEKIICQEISTLCDMLATHNGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLS
  KDSLVDLVPWLKIFPNKTLEKLKSHVKIRNDLLNKILENYKEKFRSDSITNMLDTLMQAKMNSDNGNAGP
  DQDSELLSDNHILTTIGDIFGAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQNVGFSRTPTISDRNRL
  LLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEVIINLWALHHNEKEWHQPDQFMPERFLNP
  AGTQLISPSVSYLPFGAGPRSCIGEILARQELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFK
  VKIKVRQAWREAQAEGST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000102
• ORF Size: 1524 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000102.4
• RefSeq Size: 1755 bp
• RefSeq ORF: 1527 bp
• Locus ID: 1586
• UniProt ID: P05093
• Cytogenetics: 10q24.32
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: C21-Steroid hormone metabolism, Metabolic pathways
• MW: 57.2 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]