Caspase 3 (CASP3) (NM_032991) Human Untagged Clone

CAT#: SC101958

CASP3 (untagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Caspase 3
• Synonyms: CPP32; CPP32B; SCA-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101958 sequence for NM_032991 edited (data generated by NextGen Sequencing)
  ATGGAGAACACTGAAAACTCAGTGGATTCAAAATCCATTAAAAATTTGGAACCAAAGATC
  ATACATGGAAGCGAATCAATGGACTCTGGAATATCCCTGGACAACAGTTATAAAATGGAT
  TATCCTGAGATGGGTTTATGTATAATAATTAATAATAAGAATTTTCATAAAAGCACTGGA
  ATGACATCTCGGTCTGGTACAGATGTCGATGCAGCAAACCTCAGGGAAACATTCAGAAAC
  TTGAAATATGAAGTCAGGAATAAAAATGATCTTACACGTGAAGAAATTGTGGAATTGATG
  CGTGATGTTTCTAAAGAAGATCACAGCAAAAGGAGCAGTTTTGTTTGTGTGCTTCTGAGC
  CATGGTGAAGAAGGAATAATTTTTGGAACAAATGGACCTGTTGACCTGAAAAAAATAACA
  AACTTTTTCAGAGGGGATCGTTGTAGAAGTCTAACTGGAAAACCCAAACTTTTCATTATT
  CAGGCCTGCCGTGGTACAGAACTGGACTGTGGCATTGAGACAGACAGTGGTGTTGATGAT
  GACATGGCGTGTCATAAAATACCAGTGGAGGCCGACTTCTTGTATGCATACTCCACAGCA
  CCTGGTTATTATTCTTGGCGAAATTCAAAGGATGGCTCCTGGTTCATCCAGTCGCTTTGT
  GCCATGCTGAAACAGTATGCCGACAAGCTTGAATTTATGCACATTCTTACCCGGGTTAAC
  CGAAAGGTGGCAACAGAATTTGAGTCCTTTTCCTTTGACGCTACTTTTCATGCAAAGAAA
  CAGATTCCATGTATTGTTTCCATGCTCACAAAAGAACTCTATTTTTATCACTAA
  
  Clone variation with respect to NM_032991.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032991 unedited
  TTCAGCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCGGTT
  GTGGAAGAGTTTCGTGAGTGCTCGCAGCTCATACCTGTGGCTGTGTATCCGTGGCCACAG
  CTGGTTGGCGTCGCCTTGAAATCCCAGGCCGTGAGGAGTTAGCGAGCCCTGCTCACACTC
  GGCGCTCTGGTTTTCGGTGGGTGTGCCCTGCACCTGCCTCTTCCCCCATTCTCATTAATA
  AAGGTATCCATGGAGAACACTGAAAACTCAGTGGATTCAAAATCCATTAAAAATTTGGAA
  CCAAAGATCATACATGGAAGCGAATCAATGGACTCTGGAATATCCCTGGACAACAGTTAT
  AAAATGGATTATCCTGAGATGGGTTTATGTATAATAATTAATAATAAGAATTTTCATAAA
  AGCACTGGAATGACATCTCGGTCTGGTACAGATGTCGATGCAGCAAACCTCAGGGAAACA
  TTCAGAAACTTGAAATATGAAGTCAGGAATAAAAATGATCTTACACGTGAAGAAATTGTG
  GAATTGATGCGTGATGTTTCTAAAGAAGATCACAGCAAAAGGAGCAGTTTTGTTTGTGTG
  CTTCTGAGCCATGGTGAAGAAGGAATAATTTTTGGAACAAATGGACCTGTTGACCTGAAA
  AAAATAACAAACTTTTTCAGAGGGGATCGTTGTAGAAGTCTAACTGGAAAACCCAAACTT
  TTCATTATTCAGGCCTGCCGTGGTACAGAACTGGACTGTGGCATTGAGACAGACAGTGGT
  GTTGATGATGACATGGCGTGTCATAAATACCAGTGGNAGCCGACTTCTGTATGCATACTC
  ACAGCACTGGTTATTATCTNGGCGAATTCAAGGAGGCTCCTGGNTCTCCAGTCGCTTGTG
  CCTGCTGAAACATATGCCGACAGCTTGAATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032991 unedited
  GGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTTTGAAT
  GTATATTTTGAAATAAAAATGGGAAAATAACAAATTTGTACAAAATTGTCACATTAGAAA
  CACACTTTAAGATACCATTACATGCTATGTGTATTTACAAAAGTTACAGGTCTAGTAAGA
  AAAAAGAAACTGTCATTGACAAATGCGAGCTCATTTTTTGAGCAACAAGAAATCTCCCGT
  GAAATGTCATACTGACAGCCAGTGAGACTTGGTGCAGTGACGGCTCCGCACCTGCTGAGG
  CCTGGAGCCTGCCTCCCGGGCTGAGTGCTCAGCTCTGGCCTCCGGCTGGATGCCGTCTAA
  AGTCCTATGTGCTCATAGGTCTTTTTCTTTCAGTAAACAGTATAAAACATTACTATTTTC
  CTTTGATTGGAAACTTCAACAGAAAATAACATACTTAAGAATTTTGCTACAAAGAGTACT
  ATGATTTTTATTGCCTCACCACCTTTAGAACATTTCCACTTCACAAGTTTCTGATTTGCT
  TCACTTTCTTACTTGGCGATGGCGCCCTGGCAGCATCATCCACACATACCAGTGCGTATG
  GAGAAATGGGCTGTAGGCCCTTCCTGCGTGGTCCATGGCTCTGCCTTCATGGAACAGGCT
  GAGTCATGTCTCTGCTCAAGCTCAAACCATCTACTCTCAAGTTACAGGCAGCTCAGCCCA
  TTCATTTATTGCTTTCCTTATATTCCACTGTTTATTATTTAAAGTGTATTTTCATATGTN
  CCTATAGAAATTATTCACATTACCTGGCTAATAATCACAGTTTGGGGTTTTTATATAATA
  GCTCTTCAGAATATTGCTGGGTTATGAAGCTCACTGATATGCTAGGGACAATANAANATT
  TATGCTGTAGATCAAGGCATGTACACCTCAGTAAACTCACCTTATGATGTAGTTACCTTA
  AGCAGTATCTGCCACTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032991
• Insert Size: 2680 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032991.2, NP_116786.1
• RefSeq Size: 2522 bp
• RefSeq ORF: 834 bp
• Locus ID: 836
• UniProt ID: P42574
• Cytogenetics: 4q35.1
• Domains: CASc, ICE_p10, ICE_p20
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis
• Gene Summary: The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (2, also known as beta) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a).