Caspase 3 (CASP3) (NM_032991) Human Untagged Clone

CAT#: SC322483

CASP3 (untagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Caspase 3
• Synonyms: CPP32; CPP32B; SCA-1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322483 CGGATGGGTGCTATTGTGAGGCGGTTGTGGAAGAGTTTCGTGAGTGCTCGCAGCTCATAC
  CTGTGGCTGTGTATCCGTGGCCACAGCTGGTTGGCGTCGCCTTGAAATCCCAGGCCGTGA
  GGAGTTAGCGAGCCCTGCTCACACTCGGCGCTCTGGTTTTCGGTGGGTGTGCCCTGCACC
  TGCCTCTTCCCCCATTCTCATTAATAAAGGTATCCATGGAGAACACTGAAAACTCAGTGG
  ATTCAAAATCCATTAAAAATTTGGAACCAAAGATCATACATGGAAGCGAATCAATGGACT
  CTGGAATATCCCTGGACAACAGTTATAAAATGGATTATCCTGAGATGGGTTTATGTATAA
  TAATTAATAATAAGAATTTTCATAAAAGCACTGGAATGACATCTCGGTCTGGTACAGATG
  TCGATGCAGCAAACCTCAGGGAAACATTCAGAAACTTGAAATATGAAGTCAGGAATAAAA
  ATGATCTTACACGTGAAGAAATTGTGGAATTGATGCGTGATGTTTCTAAAGAAGATCACA
  GCAAAAGGAGCAGTTTTGTTTGTGTGCTTCTGAGCCATGGTGAAGAAGGAATAATTTTTG
  GAACAAATGGACCTGTTGACCTGAAAAAAATAACAAACTTTTTCAGAGGGGATCGTTGTA
  GAAGTCTAACTGGAAAACCCAAACTTTTCATTATTCAGGCCTGCCGTGGTACAGAACTGG
  ACTGTGGCATTGAGACAGACAGTGGTGTTGATGATGACATGGCGTGTCATAAAATACCAG
  TGGAGGCCGACTTCTTGTATGCATACTCCACAGCACCTGGTTATTATTCTTGGCGAAATT
  CAAAGGATGGCTCCTGGTTCATCCAGTCGCTTTGTGCCATGCTGAAACAGTATGCCGACA
  AGCTTGAATTTATGCACATTCTTACCCGGGTTAACCGAAAGGTGGCAACAGAATTTGAGT
  CCTTTTCCTTTGACGCTACTTTTCATGCAAAGAAACAGATTCCATGTATTGTTTCCATGC
  TCACAAAAGAACTCTATTTTTATCACTAAAGAAATGGTTGGTTGGTGGTTTTTTTTAGTT
  TGTATGCCAAGTGAGAAGATGGTATATTTGGTACTGTATTTCCCTCTCATTTTGACCTAC
  TCTCATGCTGCAGAGGGTACTTTAAGACATACTCCTTCCATCAAATAGAACCACTATGAA
  GCTACCTCAAACTTCCAGTCAGGTAGTTGCAATTGAATTAAATTAGGAATAAATAAAAAT
  GGATACTGGTGCAGTCATTATGAGAGGCAATGATTGTTAATTTACAGCTTTCATGATTAG
  CAAGTTACAGTGATGCTGTGCTATGAATTTTCAAGTAATTGTGAAAAAGTTAAACATTGA
  AGTAATGAATTTTTATGATATTCCCCCCACTTAAGACTGTGTATTCTAGTTTTGTCAAAC
  TGTAGAAATGATGATGTGGAAGAACTTAGGCATCTGTGGGCATGGTCAAAGGCTCAAACC
  TTTATTTTAGAATTGATATACACGGATGACTTAACTGCATTTTTAGACCATTTATCTGGG
  ATTATGGTTTTGTGATGTTTGTCCTGAACACTTTTGTTGTAAAAAAATAATAATAATGTT
  TAATATTGAGAAAGAAACTAATATTTTATGTGAGAGAAAGTGTGAGCAAACTAACTTGAC
  TTTTAAGGCTAAAACTTAACATTCATAGAGGGGTGGAGTTTTAACTGTAAGGTGCTACAA
  TGCCCCTGGATCTACCAGCATAAATATCTTCTGATTTGTCCCTATGCATATCAGTTGAGC
  TTCATATACCAGCAATATATCTGAAGAGCTATTATATAAAAACCCCAAACTGTTGATTAT
  TAGCCAGGTAATGTGAATAAATTCTATAGGAACATATGAAAATACAACTTAAATAATAAA
  CAGTGGAATATAAGGAAAGCAATAAATGAATGGGCTGAGCTGCCTGTAACTTGAGAGTAG
  ATGGTTTGAGCCTGAGCAGAGACATGACTCAGCCTGTTCCATGAAGGCAGAGCCATGGAC
  CACGCAGGAAGGGCCTACAGCCCATTTCTCCATACGCACTGGTATGTGTGGATGATGCTG
  CCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAACTTGTGAAGTGGAAATG
  TTCTAAAGGTGGTGAGGCAATAAAAATCATAGTACTCTTTGTAGCAAAATTCTTAAGTAT
  GTTATTTTCTGTTGAAGTTTACAATCAAAGGAAAATAGTAATGTTTTATACTGTTTACTG
  AAAGAAAAAGACCTATGAGCACATAGGACTCTAGACGGCATCCAGCCGGAGGCCAGAGCT
  GAGCCCTCAGCCCGGGAGGCAGGCTCCAGGCCTCAGCAGGTGCGGAGCCGTCACTGCACC
  AAGTCTCACTGGCTGTCAGTATGACATTTCACGGGAGATTTCTTGTTGCTCAAAAAATGA
  GCTCGCGTTTGTCAATGACAGTTTCTTTTTTCTTACTAGACCTGTAACTTTTGTAAATAC
  ACATAGCATGTAATGGTATCTTAAAGTGTGTTTCTATGTGACAATTTTGTACAAATTTGT
  TATTTTCCATTTTTATTTCAAAATATACATTCAAACTTAAAATTAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_032991
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032991.2, NP_116786.1
• RefSeq Size: 2522 bp
• RefSeq ORF: 834 bp
• Locus ID: 836
• UniProt ID: P42574
• Cytogenetics: 4q35.1
• Domains: CASc, ICE_p10, ICE_p20
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis
• Gene Summary: The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (2, also known as beta) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a).