Mirna primer for qPCR
qSTAR MicroRNA Primer Pairs for SYBR Green qPCR are designed to function in OriGene’s miRNA detection system. All primers are pre-validated in an expression profile experiment against normal and ovarian cancer samples. (Validation data)
- Validated and guaranteed to provide reliable data
- Detects miRNA in all types of RNA samples including enriched or degraded samples
- Designed to function in OriGene’s miRNA detection system
![Survivin.jpg](https://cdn.origene.com/images/expression/survivin.jpg)
See miRNA Primer Panels for genomewide coverage.
Get each miRNA primer pair with matching miRNA Template Standard for quantification down to absolute copy number.
Catalog No. | Description |
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HP3xxxxx or MP3xxxxx or RP3xxxxx | Lyophilized miRNA primer pair (200 reactions at 25 uL per reaction) for use in SYBR Green qPCR experiment |
Required Component of miRNA Detection Assay
First-strand cDNA Synthesis Kit—qSTAR first-strand cDNA synthesis kit for miRNA is a two-step protocol kit.
- Addition of poly (A) tail to RNA sample
- Use of anchor linker oligo dT to synthesize first-strand cDNA.
The miRNA cDNA then becomes an effective qPCR template for SYBR Green I qPCR. The kit is designed to function only with OriGene’s miRNA detection system.
Catalog No. | Description | Data Sheet |
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HP100041 | First-strand cDNA Synthesis kit for miRNA—10 reactions | |
HP100042 | First-strand cDNA Synthesis kit for miRNA—50 reactions |