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POLR3A (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

POLR3A (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

POLR3A - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro

POLR3A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Special offer: Get $100/€100 off this product. Use code: SR100

Lenti-ORF clone of POLR3A (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A)

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti-ORF clone of POLR3A (mGFP-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A)

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, POLR3A (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, POLR3A (mGFP-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A), 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

POLR3A CRISPRa kit - CRISPR gene activation of human RNA polymerase III subunit A

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

(untagged)-Human cDNA: FLJ21091 fis, clone CAS03642

Vector pCMV6-XL5
Tag Tag Free
Mammalian Cell Selection None

POLR3A (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A)

Vector pCMV6-XL4
Tag Tag Free
Mammalian Cell Selection None

qPCR primer pairs and template standards against Homo sapiens gene POLR3A

Application Plasmid of exact quantity for transcript copy number calculation

qSTAR qPCR primer pairs against Homo sapiens gene POLR3A

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.

3' UTR clone of polymerase (RNA) III (DNA directed) polypeptide A 155kDa (POLR3A) for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

POLR3A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin