Gene Expression

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BMI1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro

qSTAR qPCR primer pairs against Homo sapiens gene BMI1

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.

BMI1 CRISPRa kit - CRISPR gene activation of human BMI1 proto-oncogene, polycomb ring finger

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

qPCR primer pairs and template standards against Homo sapiens gene BMI1

Application Plasmid of exact quantity for transcript copy number calculation