Gene Expression

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Adora3 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro

Adora3 - mouse gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro

ADORA3 CRISPRa kit - CRISPR gene activation of human adenosine A3 receptor

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Adora3 CRISPRa kit - CRISPR gene activation of mouse adenosine A3 receptor

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Adora3 - mouse gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA mBFP-Neo

Adora3 - mouse gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA Luciferase-Puro

Adora3 - mouse gene knockout kit via CRISPR, HDR mediated

Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA RFP-BSD

qSTAR qPCR primer pairs against Homo sapiens gene ADORA3

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.

qPCR primer pairs and template standards against Homo sapiens gene ADORA3

Application Plasmid of exact quantity for transcript copy number calculation

qSTAR qPCR primer pairs against Mus musculus gene Adora3

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.

qPCR primer pairs and template standards against Homo sapiens gene ADORA3

Application Plasmid of exact quantity for transcript copy number calculation