Antibodies

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Goat IgG (H+L chain) Chicken Polyclonal Antibody

AP31796PU-N

USD 270.00

2 Weeks

Goat IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications	ELISA. Western Blot (1/1000). Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities	Goat
Conjugation	Unconjugated
Immunogen	Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (whole protein).

Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

AP31795HR-N

USD 340.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Applications	Sandwich ELISA: 1/120,000 dilution of antibody was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate. Western blot (1/5000). Immunoelectrophoresis.
Reactivities	Chicken
Conjugation	HRP
Immunogen	Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.

Goat IgG (H+L chain) Chicken Polyclonal Antibody

AP31796HR-N

USD 340.00

2 Weeks

Goat IgG (H+L chain) chicken polyclonal antibody, HRP

Applications	ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis.
Reactivities	Goat
Conjugation	HRP
Immunogen	Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.

Human IgG (H+L chain) Chicken Polyclonal Antibody

AP31797PU-N

USD 270.00

2 Weeks

Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications	ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities	Human
Conjugation	Unconjugated
Immunogen	Purified, non-denatured Human IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains).

Rabbit IgG (H+L chain) Chicken Polyclonal Antibody

AP31799PU-N

USD 270.00

2 Weeks

Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications	ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities	Rabbit
Conjugation	Unconjugated
Immunogen	Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains).

Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

AP31795PU-N

USD 280.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified

Applications	ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution). Western blot (1/5000). Immunocytochemistry. Immunohistochemistry (1/250). Immunoprecipitation (1/250). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities	Chicken
Conjugation	Unconjugated
Immunogen	Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.

Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

AP31795BT-N

USD 380.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin

Applications	ELISA. Western Blot (1/5000). Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate.
Reactivities	Chicken
Conjugation	Biotin
Immunogen	Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.

Mouse IgG (H+L chain) Chicken Polyclonal Antibody

AP31798HR-N

USD 335.00

2 Weeks

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications	ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot: 1/200 -1/5000. Immunocytochemistry. Immunohistochemistry: 1/250 - 1/5000. Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities	Mouse
Conjugation	HRP
Immunogen	Purified, non-denatured Mouse IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase.

Rabbit IgG (H+L chain) Chicken Polyclonal Antibody

AP31799FC-N

USD 290.00

2 Weeks

Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC

Applications	Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities	Rabbit
Conjugation	FITC
Immunogen	Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate.

Rabbit IgG (H+L chain) Chicken Polyclonal Antibody

AP31799HR-N

USD 340.00

2 Weeks

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications	ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities	Rabbit
Conjugation	HRP
Immunogen	Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated to Horseradish Peroxidase.

PrecipHen® (Chicken IgY) Goat Polyclonal Antibody

AP31795AG-N

USD 670.00

2 Weeks

PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose

Applications	Immunoprecipitation.
Reactivities	Chicken
Conjugation	Agarose
Immunogen	Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Production: Goat anti-Chicken IgY antibodies were affinity purified from the sera over a Chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated Agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50mM HEPES buffer (pH 7.6) with 5mM EDTA (TE buffer). Sodium Azide was then added to a final concentration of 0.02% (w/v).

Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

AP31795AP-N

USD 305.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications	ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate. Western Blot (1/1000). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
Reactivities	Chicken
Conjugation	AP
Immunogen	Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.

Human IgG (H+L chain) Chicken Polyclonal Antibody

AP31797FC-N

USD 290.00

2 Weeks

Human IgG (H+L chain) chicken polyclonal antibody, FITC

Applications	Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities	Human
Conjugation	FITC
Immunogen	Purified, non-denatured Human IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate.

Mouse IgG (H+L chain) Chicken Polyclonal Antibody

AP31798FC-N

USD 290.00

2 Weeks

Mouse IgG (H+L chain) chicken polyclonal antibody, FITC

Applications	Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities	Mouse
Conjugation	FITC
Immunogen	Purified, non-denatured Mouse IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Fluorescein Isothiocyanate.

Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

AP31795FC-N

USD 285.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications	Immunocytochemistry (1/200). In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities	Chicken
Conjugation	FITC
Immunogen	Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.

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