IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified
| Applications | ELISA: 1/1000-1/2000 (2-9). Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11). Immunohistochemistry on Paraffin Sections: reported in literature (10). Flow Cytometry: reported in literature (15, 18, 19). Immunocytochemistry: reported in literature (12). Western Blot: reported in literature (3,13-16). |
| Reactivities | Human |
| Conjugation | AP |
USD 245.00
2 Weeks
Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
| Conjugation | Unconjugated |
Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified
| Applications | Western blot. Recommended dilution: 1 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining. Immunocytochemistry. ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
USD 440.00
3 Days
Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE
| Applications | Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells. FLISA: < / = 1.0 µg/ml |
| Reactivities | Rat |
| Conjugation | PE |
USD 205.00
2 Weeks
Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, Aff - Purified
| Applications | Immunocytochemistry. Western Blotting: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM. Flow cytometry: 1-4 μg/ml. ELISA: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments by ELISA. The antibody EM-07 does not crossreact with IgM. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP
| Applications | ELISA: 1/4,000-1/10,000. Western blotting. Dot- and Slot-immunoblotting. Immunohistochemistry on Frozen Sections: 1/10-1/20. |
| Reactivities | Human |
| Conjugation | HRP |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Germany
Japan
United Kingdom
China
