IL1RAPL1 Human shRNA Plasmid Kit (Locus ID 11141)

CAT#: TL312177

IL1RAPL1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL312177A virus into HEK293 cells. TL312177A virus was prepared using lenti-shRNA TL312177A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL312177B virus into HEK293 cells. TL312177B virus was prepared using lenti-shRNA TL312177B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL312177C] virus into HEK293 cells. [TL312177C] virus was prepared using lenti-shRNA [TL312177C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL312177D] virus into HEK293 cells. [TL312177D] virus was prepared using lenti-shRNA [TL312177D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 11141
• Synonyms: IL-1-RAPL-1; IL-1RAPL-1; IL1R8; IL1RAPL; IL1RAPL-1; MRX10; MRX21; MRX34; OPHN4; TIGIRR-2
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: IL1RAPL1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 11141). 5µg purified plasmid DNA per construct
  29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_014271, NM_014271.1, NM_014271.2, NM_014271.3, BC111918, BC126345, BC126347, NM_014271.4
• UniProt ID: Q9NZN1
• Summary: The protein encoded by this gene is a member of the interleukin 1 receptor family and is similar to the interleukin 1 accessory proteins. This protein has an N-terminal signal peptide, three extracellular immunoglobulin Ig-like domains, a transmembrane domain, an intracellular Toll/IL-1R domain, and a long C-terminal tail which interacts with multiple signalling molecules. This gene is located at a region on chromosome X that is associated with a non-syndromic form of X-linked intellectual disability. Deletions and mutations in this gene were found in patients with intellectual disability. This gene is expressed at a high level in post-natal brain structures involved in the hippocampal memory system, which suggests a specialized role in the physiological processes underlying memory and learning abilities, and plays a role in synapse formation and stabilization. [provided by RefSeq, Jul 2017]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).