TTC35 (EMC2) (NM_014673) Human Recombinant Protein
CAT#: TP761760
Purified recombinant protein of Human tetratricopeptide repeat domain 35 (TTC35), full length, with N-terminal His tag, expressed in E. coli, 50ug
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
A DNA sequence encoding human full-length TTC35
|
Tag | N-His |
Predicted MW | 34.7 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 50 mM Tris-HCl, pH 8.0, 8 M urea |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_055488 |
Locus ID | 9694 |
UniProt ID | Q15006 |
Cytogenetics | 8q23.1 |
Refseq Size | 1268 |
Refseq ORF | 891 |
Synonyms | KIAA0103; TTC35 |
Summary | Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).[UniProtKB/Swiss-Prot Function] |
Protein Families | Protease |
Documents
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC415127 | EMC2 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 134.00 |
|
LY415127 | Transient overexpression lysate of tetratricopeptide repeat domain 35 (TTC35) |
USD 436.00 |
|
PH304770 | TTC35 MS Standard C13 and N15-labeled recombinant protein (NP_055488) |
USD 3,255.00 |
|
TP304770 | Recombinant protein of human tetratricopeptide repeat domain 35 (TTC35), 20 µg |
USD 867.00 |
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