CD16 (FCGR3A) Human Recombinant Protein
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Specifications
Product Data | |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence |
Gly17-Gln208
|
Tag | C-His |
Predicted MW | The protein has the predicted molecular weight of 24 kDa and migrates at approximately 40-50 kDa on SDS-PAGE with DTT-reduced condition before PE conjugation. |
Concentration | 25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration. |
Purity | >90% |
Conjugation | PE |
Presentation | Purified |
Buffer | 1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein |
Bioactivity | PositiveThe definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test. |
Preparation | Affinity Ni-NTA |
Applications | FACS |
Storage | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. |
Stability | 6 Months |
Reference Data | |
RefSeq | NP_001121068 |
Locus ID | 2214 |
UniProt ID | P08637 |
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FAQs |
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Resources
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