CD16 (FCGR3A) Human Recombinant Protein
Specifications
Product Data | |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence |
Gly17-Gln208
|
Tag | C-His-Avi |
Predicted MW | The protein has the predicted molecular weight of 25.5 kDa and migrates at approximately 40-50 kDa on SDS-PAGE with DTT-reduced condition. |
Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration. |
Purity | >90% |
Presentation | Purified |
Buffer | 1xPBS buffer, pH7.4 |
Bioactivity | PositiveThe definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test. |
Preparation | Affinity Ni-NTA |
Applications | ELISA |
Storage | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. |
Stability | 6 Months |
Reference Data | |
RefSeq | NP_001121068 |
Locus ID | 2214 |
UniProt ID | P08637 |
Summary | CD16a (FCGR3A) and its homolog CD16b are receptors for the Fc portion of human IgG. In contrast to CD64, which is a high affinity Fc binding protein, both CD16a and CD16b can bind Fc portion of human IgG with relative low affinity. Human CD16a is expressed mainly on natural killer cells, macrophages, T cells, and monocytes. While human CD16b is expressed on Neutrophils and eosinophils. In human cells, a single nucleotide polymorphism (T230A) creates a high binding (176V) and a low binding (176F) variant. Clinically, mutations in CD16a have been linked to vulnerability to viral infections, alloimmune neonatal neutropenia, and systemic lupus erythematosus. |
Documents
FAQs |
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Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC400194 | FCGR3A HEK293T cell transient overexpression lysate (as WB positive control) |
USD 134.00 |
|
LC426815 | FCGR3A HEK293T cell transient overexpression lysate (as WB positive control) |
USD 134.00 |
|
LC426817 | FCGR3A HEK293T cell transient overexpression lysate (as WB positive control) |
USD 134.00 |
|
LY400194 | Transient overexpression lysate of Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1 |
USD 436.00 |
|
LY426815 | Transient overexpression lysate of Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 3 |
USD 436.00 |
|
LY426817 | Transient overexpression lysate of Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 5 |
USD 436.00 |
|
PH306429 | FCGR3A MS Standard C13 and N15-labeled recombinant protein (NP_000560) |
USD 3,255.00 |
|
TP306429 | Recombinant protein of human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1, 20 µg |
USD 867.00 |
|
TP721247 | Biotinylated Human CD16a Protein (C-His-Avi, 176V) |
USD 366.00 |
|
TP721248 | PE Conjugated Human CD16a Protein (C-His, 176V) |
USD 366.00 |
|
TP721249 | APC Conjugated Human CD16a Protein (C-His, 176V) |
USD 366.00 |
|
TP723977 | Human FCGR3A Protein, His Tag |
USD 495.00 |
|
TP762433 | Purified recombinant protein of Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 3, Leu13-Gln208, with N-terminal His tag, expressed in E.coli, 50ug |
USD 226.00 |
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