Rasgrf1 (NM_011245) Mouse Recombinant Protein

CAT#: TP524849

Purified recombinant protein of Mouse RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR224849 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEG
  SICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEAL
  MQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEVTITNLIKDNDRIQSSNKAGSADDEDSDIKKI
  KKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASS
  KKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQI
  LAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKL
  EELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQMSLSEKSKSSRGRLGSLSTKK
  EGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKD
  SLPFTVILVASTRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKT
  MNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTNAMVVLDKLINIYRKPMSAIPARSLEL
  LFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYA
  NIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPATIPEKPGELSASRKHSSDVLKEESEDDQNHSDED
  NTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGILMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKE
  VFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPD
  LLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFENHPALEIAEQLTLLDHLVFKSIPYEE
  FFGQGWMKAEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDISARASAIEKWVAVADICRCLHNYNAVLE
  ITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLRESLRNCDPPCVPYLGMYLTDLVFIE
  EGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIDPQPKVIQYLLDESFMLDEESLYESSLLIEPKL
  PT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 144.1 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_035375
• Locus ID: 19417
• UniProt ID: P27671, B2RS27
• Cytogenetics: 9 47.31 cM
• Refseq Size: 4243
• Refseq ORF: 3789
• Synonyms: AI844718; CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190; P190-A; p190RhoGEF; Ras-GRF1
• Summary: Promotes the exchange of Ras-bound GDP by GTP.[UniProtKB/Swiss-Prot Function]