Herc6 (NM_025992) Mouse Recombinant Protein

CAT#: TP524065

Purified recombinant protein of Mouse hect domain and RLD 6 (Herc6), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR224065 representing NM_025992
  Red=Cloning site Green=Tags(s)
  
  MYFSWAAGSRKPRRLKAGTSGIELLQAASGEHHSLLLFSNHRVYSCGDNSWGQLGQRRDQSTERPEPIQA
  LNDLHVDLVSCGKEHSVAVCHKGKVFAWGAGSEGQLGIGEFKEISFMPTKIKALAGIKIIQVSCGHYHSL
  ALSEDGHVFSWGRNSEGQLGLGKNSRSQAIPQKVKSLEGIPLAQVAAGGTHSFALSLTGTSFGWGSNRSG
  QLALSGNKVKEQIYKPHSIGALKNLSVIYISCGYEHTAVLTEEGQVFTFGGNSSGQLQPSPRSGQRGPQL
  IEGIGGRVSQIECASYHTIAYVYTTGQVVSLGRGPSHTSNPTHQEAPAENSDITCLLAAEDLVDIEVKDI
  FAGAHANFVTTRRVSHTRSTGVSMKILPEIIRINQSLVKKWRAANKRKDREGAKREISLIFSSSACLTAS
  FLKKRDAGENNLIDVDLKLARDVFKKLTTEKWISSLITTCLEEYLLRDLPYSSPHQEALLVFLLLPECSI
  MQDPKNWKTLAFEFAKAIHKMGPQSLAFLRTCWASLEVSSLNILVQMLKKAIISQIQYGVATEQYITNIK
  VLLEVIKEVHKANCQLPESAFIINELSGIFNFDAEAGRMFIRHNDLDCTESSDMVVFSDFLFVFDLPSKI
  KLMKCDSFVKLMSEVMAFPEKMSSPPYLILKVRRSHLVEDTLRQLRQVEDFDLRKQLSVGFINEIRPEAG
  GVSSEFFHCIFEEMTDPKYEMFIYPEKGSSMWFPVNPKFEKSSYFLFGILCGLSLHNLKVINLPFPLALY
  KKLLNQKPSLEDLKELSLPLGRNLQEVLNCEAGDIEELHMYFSIYWDQKDVDLIPDGISVPVNETNKRDY
  VSKYVDYIFNISIKTIYEEFHRGFYKVCNWDIIRQFQPEELMTAIIGNATCDWKQFENNSKYKDGYDKSH
  PTILLFWKAFHDLTLDEKKKFLLFLTGCDRLHVKGLQNEGIVFRCSETFSEEDNPRSLTCHRMLDLPKYS
  SMRRMKEALQVAINNSTGFVSQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 112.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_080268
• Locus ID: 67138
• UniProt ID: F2Z461
• Cytogenetics: 6
• Refseq Size: 5279
• Refseq ORF: 3009
• Synonyms: 1700121D12Rik; 2510038N07Rik; 4930427L17Rik; AI451296; CEB1; Herc5
• Summary: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon.[UniProtKB/Swiss-Prot Function]