Prag1 (NM_172911) Mouse Recombinant Protein

CAT#: TP511757

Purified recombinant protein of Mouse PEAK1 related kinase activating pseudokinase 1 (Prag1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211757 representing NM_172911
  Red=Cloning site Green=Tags(s)
  
  MSACSDFVEHIWKPGSCKNCFCLRSDHQPTAGHPKARANSLPAGTRLPARPENCRLDDEGVNGLAYSKPT
  IAVKPTMMTSETSDLWTEASLSAEVPKVNWRRTPGKLLLPKQEDEPIVYLGSFRGLQKPASPLACTDGNS
  RCPPAYTMVGLHNLEARVDRNTAFQPVSFQEEKAGREELPSAHESFRQKLAAFAGMTSSCPKGPRPCTSP
  QPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPESKDAVHSTEGSGRRGGDCSPTCREQGPRTRPTE
  EEKQGLSFPRECCGQGSTANPPRLGPKKPSLNSEAASSSDGLSCGSSRSGASSPFAPHLENDYCSLVKEP
  ASGKQQDLSGHFLTSGKCVGQAAELQPASLLRDPVQPEPIYAESAKRKKAAPGPPRPEPKKEQVPAGHSQ
  GQVWTGDTWIQKTPPSWSQDREGANPAPQVATTITVIAAHPEEDHRTIYLSSPDSAVGVQWPRGPSNQDL
  QAGEEEPLVAQGLTSRESHPHNVTENTAKEKPAIPPKLSKSSPGGSPVSPAPPLTDHSDGNTGGSSVGPQ
  LLSRVPANLTSSCHTNGVATAGDSAKCPPPATSSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQELSQSWG
  RELENGTADHSNSSTWHRLHPIDGTSGQNSKTNSGMSKSASFAFEFPKDRGRLEAFSPPPPPPKSRHLLK
  MNKSSSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLASDSRPCSDGGPSYEPTHSPTISGKKLFAPVP
  FPSGSTEDVSPGGGPAQPPPLPQKKIVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPF
  NCSLNSGNRSHHVFSSSEPLGKAFKGNAPWAPALGLANSKGGCGSPSLQCRAATSTSSSQLSVSSQASSS
  STQLQLHSLLSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGQKKELRFNENYWSLFKLTCNKPCCD
  SGDAIYYCATCSEDPGSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCGHFVASVPSSMLASPDTSSKD
  TAPAVSPQPPAQEQDCVVVITREVPHQTASDFVRDSMASHRAEPEVYERRVCFLLLQLCNGLEHLKEHGI
  IHRDLCLENLLLAHCNPQSSPGPSATPTVPTTTSRCPSAAPAATTACQGGPGEKQLPRLIISNFLKAKQK
  PGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTL
  SLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCTSEEVLCNTLHNWIDMKRALMM
  MKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 148.4 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_766499
• Locus ID: 244418
• UniProt ID: Q571I4
• Cytogenetics: 8 22.78 cM
• Refseq Size: 4780
• Refseq ORF: 4119
• Synonyms: 9830148H23; D8Ertd82e; mFLJ00269; NACK; Sgk223
• Summary: Catalytically inactive protein kinase that acts as a scaffold protein (By similarity). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (PubMed:25038227).[UniProtKB/Swiss-Prot Function]