Sf3b5 (NM_175102) Mouse Recombinant Protein

CAT#: TP500186

Purified recombinant protein of Mouse splicing factor 3b, subunit 5 (Sf3b5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from Mouse cDNA ORF Clone, MR200186, encoding Mouse full-length Sf3b5.
• Tag: C-MYC/DDK
• Predicted MW: 10.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_780311
• Locus ID: 66125
• UniProt ID: Q923D4
• Cytogenetics: 10 A2
• Refseq Size: 750
• Refseq ORF: 258
• Synonyms: 10kDa; 1110005L13Rik; AU043053; Sf3b10
• Summary: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA.[UniProtKB/Swiss-Prot Function]