Idnk (1-187, His-tag) Escherichia coli Protein
Specifications
Product Data | |
Species | Escherichia coli |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
|
Tag | His-tag |
Predicted MW | 23.4 kDa |
Concentration | lot specific |
Purity | >90% by SDS - PAGE |
Presentation | Purified |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1 mM DTT |
Bioactivity | Specific: Specific activity is > 80 units/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C. |
Preparation | Liquid purified protein |
Storage | Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
Summary | D-glucote kise, thermosensitive, also known as idnk, D-glucote kise, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokise gntK/gntV family and catalyzes the conversion of ATP and D-glucote to ADP and 6-phospho-D-glucote. This protein is thought to play a role in gender determition, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombint E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventiol chromatography techniques. |
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