PIN4 (1-156, His-tag) Human Protein

CAT#: AR09938PU-N

PIN4 (1-156, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLERFSVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK
• Tag: His-tag
• Predicted MW: 18.8 kDa
• Concentration: lot specific
• Purity: >85%
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glyceril 1mM, DTT, 0.1 mM PMSF
• Bioactivity: Specific: > 300 nmoles/min/mg, defined as the amount of enzyme that cleaves
  1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin.
• Preparation: Liquid purified protein
• Applications: Protocol: Activity Assay
  1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
  The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20 nM chymotrypsin.
  2. Add 10 ul of recombinant PIN4 protein with 1 ug in assay buffer.
  3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
  4. Add 20 ul pre-chilled 5 mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460 mM LiCl to a concentration of 3 mM)
  5. Record the increase in A405 nm for 30 minutes at 25°C.
• Protein Description: Recombinant human PIN4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001164218
• Locus ID: 5303
• UniProt ID: Q9Y237
• Cytogenetics: Xq13.1
• Synonyms: EPVH; PAR14; PAR17
• Summary: This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009]