slyD (1-196) Escherichia coli Protein
Specifications
Product Data | |
Species | Escherichia coli |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
|
Predicted MW | 21 kDa |
Concentration | lot specific |
Purity | >95% by SDS PAGE |
Presentation | Purified |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris pH 7.5 |
Bioactivity | Specific: > 220 nmoles/min/mg, defined as the amount of enzyme that cleaves 1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin. |
Preparation | Liquid purified protein |
Applications | Protocol: Activity Assay 1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 2. Add 10 ul of recombinant SlyD protein with 1 ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5. Record the increase in A405 nm for 30 minutes at 25°C. |
Protein Description | Recombinant E.coli slyD was expressed in E.coli and purified by conventional chromatography. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
Summary | SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. |
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