beta 2 Microglobulin (B2M) (NM_004048) Human Over-expression Lysate
Specifications
Product Data | |
Species | Human |
Applications | WB |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC207587 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Accession Number | NM_004048, NP_004039 |
Other Names | IMD43 |
Predicted MW | 13.71 kDa |
Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT) |
Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
Reference Data | |
RefSeq | NP_004039 |
Locus ID | 567 |
Cytogenetics | 15q21.1 |
Protein Families | WB |
Protein Pathways | Antigen processing and presentation |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC401311 | B2M HEK293T cell transient overexpression lysate (as WB positive control) |
USD 134.00 |
|
PH307587 | B2M MS Standard C13 and N15-labeled recombinant protein (NP_004039) |
USD 3,255.00 |
|
TP307587 | Recombinant protein of human beta-2-microglobulin (B2M), 20 µg |
USD 867.00 |
|
TP710178 | Recombinant protein of human beta-2-microglobulin (B2M), residues 21-119aa, secretory expressed with GP67 signal peptide, with C-terminal DDK tag, expressed in sf9, 20ug |
USD 515.00 |
|
TP720425 | Recombinant protein of human beta-2-microglobulin (B2M) |
USD 330.00 |
|
TP750209 | Purified recombinant protein of human beta-2-microglobulin(B2M), Ile21-Met119, with N-terminal His tag, expressed in E.coli, 50ug |
USD 226.00 |
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