NFYC (NM_001142589) Human Mass Spec Standard

CAT#: PH326680

NFYC MS Standard C13 and N15-labeled recombinant protein (NP_001136061)

Product Images

Coomassie blue staining of purified NFYC protein (Cat# TP326680). The protein was produced from HEK293T cells transfected with NFYC cDNA clone (Cat# RC226680) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC226680
• Predicted MW: 32.6 kDa
• Protein Sequence:
  Sequence Link

  >RC226680 representing NM_001142589
  Red=Cloning site Green=Tags(s)
  
  MSTEGGFGGTSSSDAQQSLQSFWPRVMEEIRNLTVKDFRVQELPLARIKKIMKLDEDVKRNDIAMAITKF
  DQFDFLIDIVPRDELKPPKRQEEVRQSVTPAEPVQYYFTLAQQPTAVQVQGQQQGQQTTSSTTTIQPGQI
  IIAQPQQGQTTPVTMQVGEGQQVQIVQAQPQGQAQQAQSGTGQTMQVMQQIITNTGEIQQIPVQLNAGQL
  QYIRLAQPVSGTQVVQGQIQTLATNAQQITQTEVQQGQQQFSQFTDGQQLYQIQQVTMPAGQDLAQPMFI
  QSANQPSDGQAPQVTGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001136061
• RefSeq ORF: 891
• Synonyms: CBF-C; CBFC; H1TF2A; HAP5; HSM; NF-YC
• Locus ID: 4802
• UniProt ID: Q13952
• Cytogenetics: 1p34.2
• Summary: This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation