H2BC3 (NM_021062) Human Mass Spec Standard

CAT#: PH320710

HIST1H2BB MS Standard C13 and N15-labeled recombinant protein (NP_066406)

Product Images

Coomassie blue staining of purified HIST1H2BB protein (Cat# TP320710). The protein was produced from HEK293T cells transfected with HIST1H2BB cDNA clone (Cat# RC220710) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC220710
• Predicted MW: 14 kDa
• Protein Sequence:
  Sequence Link

  >RC220710 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDI
  FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_066406
• RefSeq Size: 431
• RefSeq ORF: 378
• Synonyms: H2B.1; H2B/f; H2BFF; HIST1H2BB
• Locus ID: 3018
• UniProt ID: P33778
• Cytogenetics: 6p22.2
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
• Protein Pathways: Systemic lupus erythematosus