DNAJB13 (NM_153614) Human Mass Spec Standard

CAT#: PH314119

DNAJB13 MS Standard C13 and N15-labeled recombinant protein (NP_705842)

Product Images

Coomassie blue staining of purified DNAJB13 protein (Cat# TP314119). The protein was produced from HEK293T cells transfected with DNAJB13 cDNA clone (Cat# RC214119) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC214119
• Predicted MW: 35.9 kDa
• Protein Sequence:
  Sequence Link

  >RC214119 representing NM_153614
  Red=Cloning site Green=Tags(s)
  
  MGQDYYSVLGITRNSEDAQIKQAYRRLALKHHPLKSNEPSSAEIFRQIAEAYDVLSDPMKRGIYDKFGEE
  GLKGGIPLEFGSQTPWTTGYVFHGKPEKVFHEFFGGNNPFSEFFDAEGSEVDLNFGGLQGRGVKKQDPQV
  ERDLYLSLEDLFFGCTKKIKISRRVLNEDGYSSTIKDKILTIDVKPGWRQGTRITFEKEGDQGPNIIPAD
  IIFIVKEKLHPRFRRENDNLFFVNPIPLGKALTCCTVEVRTLDDRLLNIPINDIIHPKYFKKVPGEGMPL
  PEDPTKKGDLFIFFDIQFPTRLTPQKKQMLRQALLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_705842
• RefSeq Size: 1875
• RefSeq ORF: 948
• Synonyms: CILD34; RSPH16A; TSARG5; TSARG6
• Locus ID: 374407
• UniProt ID: P59910
• Cytogenetics: 11q13.4
• Summary: This gene encodes a member of the heat shock protein 40 co-chaperone family which is produced in large amounts in the testis and is located on the radial spokes of the axoneme in human sperm flagella and other flagellar structures. The encoded protein associates with the sperm annulus, as part of the septin complex, through direct interaction with septin 4, during sperm terminal differentiation. Naturally occurring mutations in this gene are associated with primary ciliary dyskinesia and male infertility. [provided by RefSeq, Apr 2017]