PRRX1 (NM_022716) Human Mass Spec Standard

CAT#: PH310393

PRRX1 MS Standard C13 and N15-labeled recombinant protein (NP_073207)

Product Images

Coomassie blue staining of purified PRRX1 protein (Cat# TP310393). The protein was produced from HEK293T cells transfected with PRRX1 cDNA clone (Cat# RC210393) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210393
• Predicted MW: 27.3 kDa
• Protein Sequence:
  Sequence Link

  >RC210393 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTSSYGHVLERQPALGGRLDSPGNLDTLQAKKNFSVSHLLDLEEAGDMVAAQADENVGEAGRSLLESPGL
  TSGSDTPQQDNDQLNSEEKKKRKQRRNRTTFNSSQLQALERVFERTHYPDAFVREDLARRVNLTEARVQV
  WFQNRRAKFRRNERAMLANKNASLLKSYSGDVTAVEQPIVPRPAPRPTDYLSWGTASPYSAMATYSATCA
  NNSPAQGINMANSIANLRLKAKEYSLQRNQVPTVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_073207
• RefSeq Size: 3999
• RefSeq ORF: 735
• Synonyms: AGOTC; PHOX1; PMX1; PRX-1; PRX1
• Locus ID: 5396
• UniProt ID: P54821
• Cytogenetics: 1q24.2
• Summary: The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns. [provided by RefSeq, Jul 2008]
• Protein Families: Transcription Factors