AGPAT5 (NM_018361) Human Mass Spec Standard

CAT#: PH310280

AGPAT5 MS Standard C13 and N15-labeled recombinant protein (NP_060831)

Product Images

Coomassie blue staining of purified AGPAT5 protein (Cat# TP310280). The protein was produced from HEK293T cells transfected with AGPAT5 cDNA clone (Cat# RC210280) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210280
• Predicted MW: 42.1 kDa
• Protein Sequence:
  Sequence Link

  >RC210280 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLSLVLHTYSMRYLLPSVVLLGTAPTYVLAWGVWRLLSAFLPARFYQALDDRLYCVYQSMVLFFFENYT
  GVQILLYGDLPKNKENIIYLANHQSTVDWIVADILAIRQNALGHVRYVLKEGLKWLPLYGCYFAQHGGIY
  VKRSAKFNEKEMRNKLQSYVDAGTPMYLVIFPEGTRYNPEQTKVLSASQAFAAQRGLAVLKHVLTPRIKA
  THVAFDCMKNYLDAIYDVTVVYEGKDDGGQRRESPTMTEFLCKECPKIHIHIDRIDKKDVPEEQEHMRRW
  LHERFEIKDKMLIEFYESPDPERRKRFPGKSVNSKLSIKKTLPSMLILSGLTAGMLMTDAGRKLYVNTWI
  YGTLLGCLWVTIKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_060831
• RefSeq Size: 5535
• RefSeq ORF: 1092
• Synonyms: 1AGPAT5; LPAATE
• Locus ID: 55326
• UniProt ID: Q9NUQ2, A0A024R640
• Cytogenetics: 8p23.1
• Summary: This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. A pseudogene of this gene is present on the Y chromosome. [provided by RefSeq, Aug 2014]
• Protein Families: Transmembrane