SMAP1 (NM_001044305) Human Mass Spec Standard

CAT#: PH306367

SMAP1 MS Standard C13 and N15-labeled recombinant protein (NP_001037770)

Product Images

Coomassie blue staining of purified SMAP1 protein (Cat# TP306367). The protein was produced from HEK293T cells transfected with SMAP1 cDNA clone (Cat# RC206367) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC206367
• Predicted MW: 50.4 kDa
• Protein Sequence:
  Sequence Link

  >RC206367 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATRSCREKAQKLNEQHQLILSKLLREEDNKYCADCEAKGPRWASWNIGVFICIRCAGIHRNLGVHISRV
  KSVNLDQWTAEQIQCMQDMGNTKARLLYEANLPENFRRPQTDQAVEFFIRDKYEKKKYYDKNAIAITNIS
  SSDAPLQPLVSSPSLQAAVDKNKLEKEKEKKKEEKKREKEPEKPAKPLTAEKLQKKDQQLEPKKSTSPKK
  AAEPTVDLLGLDGPAVAPVTNGNTTVPPLNDDLDIFGPMISNPLPATVMPPAQGTPSAPAAATLSTVTSG
  DLDLFTEQTTKSEEVAKKQLSKDSILSLYGTGTIQQQSTPGVFMGPTNIPFTSQAPAAFQGFPSMGVPVP
  AAPGLIGNVMGQSPSMMVGMPMPNGFMGNAQTGVMPLPQNVVGHQGGMVGQMGAPQSKFGLPQAQQPQWS
  LSQMNQQMAGMSISSATPTAGFGQPSSTTAGWSGSSSGQTLSTQLWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001037770
• RefSeq Size: 3349
• RefSeq ORF: 1401
• Synonyms: SMAP-1
• Locus ID: 60682
• UniProt ID: Q8IYB5
• Cytogenetics: 6q13
• Summary: The protein encoded by this gene is similar to the mouse stromal membrane-associated protein-1. This similarity suggests that this human gene product is also a type II membrane glycoprotein involved in the erythropoietic stimulatory activity of stromal cells. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Protein Pathways: Endocytosis