TPRKB (NM_016058) Human Mass Spec Standard

CAT#: PH306008

TPRKB MS Standard C13 and N15-labeled recombinant protein (NP_057142)

Product Images

Coomassie blue staining of purified TPRKB protein (Cat# TP306008). The protein was produced from HEK293T cells transfected with TPRKB cDNA clone (Cat# RC206008) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC206008
• Predicted MW: 19.7 kDa
• Protein Sequence:
  Sequence Link

  >RC206008 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQLTHQLDLFPECRVTLLLFKDVKNAGDLRRKAMEGTIDGSLINPTVIVDPFQILVAANKAVHLYKLGKM
  KTRTLSTEIIFNLSPNNNISEALKKFGISANDTSILIVYIEEGEKQINQEYLISQVEGHQVSLKNLPEIM
  NITEVKKIYKLSSQEESIGTLLDAIICRMSTKDVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057142
• RefSeq Size: 752
• RefSeq ORF: 525
• Synonyms: CGI-121; CGI121; GAMOS5
• Locus ID: 51002
• UniProt ID: Q9Y3C4
• Cytogenetics: 2p13.1
• Summary: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:28805828). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:28805828). TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tRNA modification (PubMed:22912744, PubMed:28805828).[UniProtKB/Swiss-Prot Function]