MRPL53 (NM_053050) Human Mass Spec Standard
CAT#: PH304255
MRPL53 MS Standard C13 and N15-labeled recombinant protein (NP_444278)
USD 436.00
Specifications
Product Data | |
Tag | C-Myc/DDK |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence | RC204255 |
Predicted MW | 12.1 kDa |
Protein Sequence |
>RC204255 protein sequence
Red=Cloning site Green=Tags(s) MAAALARLGLRPVKQVRVQFCPFEKNVESTRTFLQTVSSEKVRSTNLNCSVIADVRHDGSEPCVDVLFGD GHRLIMRGAHLTALEMLTAFASHIRARDAAGSGDKPGADTGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Labeling Method | Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3 |
Storage | Store at -80°C. Avoid repeated freeze-thaw cycles. |
Stability | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Reference Data | |
RefSeq | NP_444278 |
RefSeq Size | 673 |
RefSeq ORF | 336 |
Synonyms | L53MT |
Locus ID | 116540 |
UniProt ID | Q96EL3 |
Cytogenetics | 2p13.1 |
Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC409317 | MRPL53 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 134.00 |
|
LY409317 | Transient overexpression lysate of mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein |
USD 436.00 |
|
TP304255 | Recombinant protein of human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein, 20 µg |
USD 867.00 |
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