NADK (NM_023018) Human Mass Spec Standard

CAT#: PH300544

NADK MS Standard C13 and N15-labeled recombinant protein (NP_075394)

Product Images

Coomassie blue staining of purified NADK protein (Cat# TP300544). The protein was produced from HEK293T cells transfected with NADK cDNA clone (Cat# RC200544) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200544
• Predicted MW: 49.2 kDa
• Protein Sequence:
  Sequence Link

  >RC200544 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEMEQEKMTMNKELSPDAAAYCCSACHGDETWSYNHPIRGRAKSRSLSASPALGSTKEFRRTRSLHGPCP
  VTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEK
  KVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLG
  FLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQY
  QVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICP
  HSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFES
  LAQCLHWNVRKKQAHFEEEEEEEEEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_075394
• RefSeq Size: 3244
• RefSeq ORF: 1338
• Synonyms: dJ283E3.1
• Locus ID: 65220
• UniProt ID: O95544, A0A024R058, F5GXR5
• Cytogenetics: 1p36.33
• Summary: NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM, Mar 2008]
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism